TIA1 Antibody

SKU: orb1246858

Description

TIA1 Antibody

Research Area

Cell Biology

Images & Validation

• Tested Applications: ELISA, IF, IHC, WB
• Reactivity: Human
• Predicted Reactivity: Canine, Mouse, Rat
• Application Notes:
  Peptide ELISA: antibody detection limit dilution 1:128000.Western Blot:Approx 40kDa band observed in lysates of cell line Jurkat (calculated MW of 41.8kDa according to NP_071320.1). Recommended concentration: 0.1-0.3µg/mL. Primary incubation was 1 hour. An additional band at 80kDa and a very strong 26kDa is also observed. These bands were successfully blocked by incubation with the immunising peptide.Immunohistochemistry:Paraffin embedded Human Thyroid. Recommended concentration: 3.75µg/mL.Immunofluorescence: Strong expression of the protein seen in the nucleus and cytoplasm of HeLa cells. Recommended concentration: 5µg/mL.

Key Properties

• Antibody Type: Primary Antibody
• Host: Goat
• Clonality: Polyclonal
• Immunogen: The immunogen for this antibody is: C-PKSTYESNTKQ
• Target: TIA1
• Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Form/Appearance: Liquid
• Buffer/Preservatives: Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.
• Concentration: 500 ug/mL
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

TIA1, TIA1 cytotoxic granule-associated RNA binding protein , TIA1 cytotoxic granule-associated RNA-binding protein, TIA1 protein, cytotoxic granule-associated RNA-binding protein, p40-TIA-1 (containing p15-TIA-1)

orb1246858 (0.1 ug/ml) staining of Jurkat cell lysate (35 ug protein in RIPA buffer). Detected by chemiluminescence.

orb1246858 (3.75 ug/ml) staining of paraffin embedded Human Thyroid. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.

orb1246858 Immunofluorescence analysis of paraformaldehyde fixed HeLa cells, permeabilized with 0.15% Triton. Primary incubation 1hr (5 ug/ml) followed by Alexa Fluor 488 secondary antibody (2 ug/ml), showing nuclear and some cytoplasmic staining.

Quick Database Links

Gene Symbol

TIA1

UniProt

P31483

RefSeq (Protein):NP_071320.1, NP_071505.1