Swine IgG (H&L) Antibody

• Catalog Number: orb347964
• Category: Antibodies
• Description: Swine IgG (H&L) antibody
• Clonality: Polyclonal
• Species/Host: Goat
• Isotype: IgG
• Conjugation: Unconjugated
• Reactivity: Porcine
• Form/Appearance: Liquid (sterile filtered)
• Concentration: 2.17 mg/mL
• Buffer/Preservatives: 0.01% (w/v) Sodium Azide. 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
• Purity: This product was prepared from monospecific antiserum by immunoaffinity chromatography using Swine IgG coupled to agarose beads. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Goat Serum, Swine IgG and Swine Serum.
• Immunogen: Anti-Swine IgG (H&L) was produced by repeated immunization with swine whole IgG molecule in goat.
• Tested applications: ELISA, IHC, WB
• Dilution range: ELISA: 1:20,000 - 1:100,000, IHC: 1:1,000 - 1:5,000, WB: 1:2,000 - 1:10,000
• Application notes: Anti-Swine IgG (H&L) is tested by western blot and is suitable for use in immunoelectrophoresis, western-blot, competitive western-blot, ELISA and competitive ELISA assays. Specific conditions for reactivity and signal detection should be optimized by the end user.
• Antibody Type: Secondary Antibody
• Storage: Store vial at 4° C prior to opening. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use. For extended storage aliquot contents and freeze at -20° C or below. Avoid cycles of freezing and thawing.
• Alternative names: goat Anti-Swine IgG Antibody, goat Anti Swine IgG
• Note: For research use only

Western Blot of Goat anti-Swine IgG antibody. Lane 1: Swine IgG (p/n orb2652739). Lane 2: none. Load: 100 ng per lane. Primary antibody: Swine Antibody at 1:1000 o/n at 4°C. Secondary antibody: DyLight™ 649 goat secondary antibody at 1:20000 for 30 min at RT. Block: orb348637 for 30 min at RT. Predicted/Observed size: 55 kDa, 28 kDa for Swine IgG. Other band(s): none.