STUB1 Antibody

• Catalog Number: orb1263843
• Category: Antibodies
• Description: STUB1 Antibody
• Species/Host: Rabbit
• Clonality: Polyclonal
• Tested applications: IHC-P, WB
• Predicted Reactivity: Gallus, Mouse
• Reactivity: Human
• Isotype: Rabbit Ig
• Immunogen: This STUB1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 203-231 amino acids from the C-terminal region of human STUB1.
• Concentration: batch dependent
• Dilution range: For WB starting dilution is: 1:1000For IHC-P starting dilution is: 1:50~100
• Form/Appearance: Liquid
• Conjugation: Unconjugated
• MW: 35 kDa
• Target: STUB1
• UniProt ID: Q9UNE7
• NCBI: Q9UNE7
• Storage: Store at 4°C for three months and -20°C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
• Buffer/Preservatives: Supplied in PBS with 0.09% (W/V) sodium azide.
• Alternative names: E3 ubiquitin-protein ligase CHIP, 632-, Antigen NY
• Note: For research use only
• Application notes: For WB starting dilution is: 1:1000For IHC-P starting dilution is: 1:50~100
• Expiration Date: 12 months from date of receipt.

Western Blot at 1:1000 dilution + MCF-7 whole cell lysate Lysates/proteins at 20 ug per lane.

Western blot analysis in 293 cell line and human placenta lysates (35 ug/lane).

Formalin-fixed and paraffin-embedded human hepatocarcinoma tissue reacted with CHIP (STUB1) antibody, which was peroxidase-conjugated to the secondary antibody, followed by DAB staining.

Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by AEC staining. BC = breast carcinoma; HC = hepatocarcinoma.