STIM1 antibody

• Catalog Number: orb43733
• Category: Antibodies
• Description: Mouse Monoclonal to STIM1.
• Clonality: Monoclonal
• Clone Number: CDN3H4
• Tested applications: ICC, IHC-P, IP, WB
• Reactivity: Human, Mouse, Rat
• Isotype: Mouse IgG1
• Immunogen: Synthetized peptide (C-terminal cytoplasmic part of STIM1).
• Concentration: 1 mg/ml
• Dilution range: Immunocytochemistry: Methanol-aceton fixation; positive control: HeLa human cervix carcinoma cell line.Immunohistochemistry (paraffin sections): Recommended dilution: 5 μg/ml.Western blotting: Recommended dilution: 1 μg/ml; positive control: RBL rat basophilic leukemia cell line; both reducing and non-reducing conditions.
• Purity: Purified by protein-A affinity chromatography.
• Conjugation: Unconjugated
• Target: STIM1
• Entrez: 6786
• UniProt ID: G0XQ39
• Storage: Store at 2-8°C. Do not freeze.
• Buffer/Preservatives: Phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide
• Alternative names: STIM1 antibody, anti-GOK
• Note: For research use only
• Application notes: Immunocytochemistry: Methanol-aceton fixation; positive control: HeLa human cervix carcinoma cell line.Immunohistochemistry (paraffin sections): Recommended dilution: 5 μg/ml.Western blotting: Recommended dilution: 1 μg/ml; positive control: RBL rat basophilic leukemia cell line; both reducing and non-reducing conditions.
• Expiration Date: 12 months from date of receipt.

Western blotting analysis (non-reducing conditions) of whole cell lysate of RBL rat basophilic leukemia cell line. Lane 1: immunostaining with anti-STIM1 (CDN3H4); Lane 2: immunostaining with Isotype mouse IgG1 control (PPV-06; cat. no. orb43531).

Immunocytochemistry staining of HeLa human cervix carcinoma cell line using anti-STIM1 (CDN3H4; methanol-aceton fixation; detection by Goat anti-mouse IgG1 Alexa Fluor® 598; red). Nuclei were stained with DAPI (blue).