STIM1 Antibody

• Catalog Number: orb251589
• Category: Antibodies
• Description: STIM1 Antibody
• Species/Host: Rabbit
• Clonality: Polyclonal
• Tested applications: FC, ICC, IF, IHC, WB
• Predicted Reactivity: Hamster
• Reactivity: Human, Mouse, Rat
• Isotype: Rabbit IgG
• Immunogen: A synthetic peptide corresponding to a sequence at the N-terminus of human STIM1 (45-74aa AAEFCRIDKPLCHSEDEKLSFEAVRNIHKL), identical to the related mouse and rat sequences.
• Concentration: Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.
• Dilution range: Western blot, 0.1-0.5μg/ml, Human, Mouse, Rat Immunohistochemistry (Paraffin-embedded Section), 0.5-1μg/ml, Human, Mouse, Rat, By Heat Immunocytochemistry/Immunofluorescence, 5μg/ml, Human Flow Cytometry, 1-3μg/1x106 cells, Human
• Form/Appearance: Lyophilized
• Conjugation: Unconjugated
• MW: 77423 MW
• UniProt ID: Q13586
• Storage: Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.
• Alternative names: Stromal interaction molecule 1;STIM1;GOK;
• Note: For research use only
• Application notes: Tested Species: In-house tested species with positive results. By Heat: Boiling the paraffin sections in 10mM citrate buffer, pH6.0, for 20mins is required for the staining of formalin/paraffin sections. Other applications have not been tested. Optimal dilutions should be determined by end users. . Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
• Expiration Date: 12 months from date of receipt.

WB analysis of STIM1 using anti-STIM1 antibody (Left) and anti-STIM1 antibody (Right).Lane 1:human Jurakt cell;2:human K562 cell;3:human HepG2 cell;4:rat liver tissue;5:rat RH35 cell;6:mouse liver tissue.

IF analysis of STIM1 using anti-STIM1 antibody. STIM1 was detected in immunocytochemical section of A549 cells.

IHC analysis of STIM1 using anti-STIM1 antibody. STIM1 was detected in a paraffin-embedded section of mouse intestine tissue.

IHC analysis of STIM1 using anti-STIM1 antibody. STIM1 was detected in a paraffin-embedded section of rat intestine tissue.

IHC analysis of STIM1 using anti-STIM1 antibody. STIM1 was detected in a paraffin-embedded section of human mammary cancer tissue.