STAT1 Antibody

• Catalog Number: orb1240037
• Category: Tools
• Description: STAT1 Antibody
• Species/Host: Rabbit
• Clonality: Polyclonal
• Tested applications: ELISA, ICC, IF, IHC-P, IP, WB
• Isotype: IgG
• Immunogen: Anti-STAT1 alpha antibody (orb1240037) was raised against a peptide corresponding to 38 amino acids near the carboxy terminus of human STAT1 alpha. The immunogen is located within the last 50 amino acids of STAT1 alpha.
• Concentration: 1 mg/ml
• Dilution range: WB: 0.5-1 μg/mL; IF: 20 μg/mL; IHC: 2.5 μg/mL; ICC: 10 μg/mL .Antibody validated: Western Blot in human, mouse and rat samples; Immunofluorescence, Immunohistochemistry and Immunocytochemistry in human samples. All other applications and species not yet tested.
• Form/Appearance: Liquid
• Conjugation: Unconjugated
• MW: Predicted: 87kDObserved: 87 kD
• Target: STAT1
• UniProt ID: P42224
• NCBI: NP_009330
• Storage: STAT1 alpha antibody can be stored at 4°C for three months and -20°C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
• Buffer/Preservatives: STAT1 alpha Antibody is supplied in PBS containing 0.02% sodium azide.
• Alternative names: STAT1 alpha Antibody: CANDF7, ISGF-3, STAT91
• Note: For research use only
• Expiration Date: 12 months from date of receipt.

Western Blot Validation in Human and Mouse Cell Lines of (H) Hela, (J) Jurkat, (A) A431, (K) K562 and (N) 3T3/NIH. Loading: 15 µg of lysates per lane. Antibodies: STAT1 alpha orb1240037 (1 µg/mL), 1h incubation at RT in 5% NFDM/TBST. Secondary: Goat anti-rabbit IgG HRP conjugate at 1:10000 dilution.

Independent Antibody Validation (IAV) via Protein Expression Profile in Human Cell Lines. Loading: 15 µg of lysates per lane. Antibodies: STAT1 alpha orb1240037 (1 µg/mL), competitor antibody (2 µg/mL), and beta-actin (1.5 µg/mL), 1h incubation at RT in 5% NFDM/TBST. Secondary: Goat anti-rabbit IgG HRP conjugate at 1:10000 dilution.

Western Blot Validation in Mouse tissues. Loading: 15 µg of lysates per lane. Antibodies: STAT1 alpha orb1240037 (0.5 µg/mL), 1h incubation at RT in 5% NFDM/TBST. Secondary: Goat anti-rabbit IgG HRP conjugate at 1:10000 dilution.

Western Blot Validation in Rat Cell Line. Loading: 15 µg of lysates per lane. Antibodies: STAT1 alpha orb1240037 (1 µg/mL), 1h incubation at RT in 5% NFDM/TBST. Secondary: Goat anti-rabbit IgG HRP conjugate at 1:10000 dilution.

Immunofluorescence Validation of STAT1 alpha in Human Colon Tissue. Immunofluorescent analysis of 4% paraformaldehyde-fixed Human Colon Tissue labeling STAT1 alpha with orb1240037 at 20 µg/mL, followed by goat anti-rabbit IgG secondary antibody at 1/500 dilution (green) and DAPI staining (blue).

Immunohistochemistry Validation of STAT1 alpha in Human Colon Tissue. Immunohistochemical analysis of paraffin-embedded Human Colon Tissue using anti-STAT1 alpha antibody (orb1240037) at 2.5 µg/ml. Tissue was fixed with formaldehyde and blocked with 10% serum for 1 h at RT; antigen retrieval was by heat mediation with a citrate buffer (pH6). Samples were incubated with primary antibody overnight at 4°C. A goat anti-rabbit IgG H&L (HRP) at 1/250 was used as secondary. Counter stained with Hematoxylin.

Immunocytochemistry Validation of STAT1 alpha in Human HeLa Cells. Immunocytochemical analysis of HeLa cells using anti-STAT1 alpha antibody (orb1240037) at 10 µg/ml. Cells was fixed with formaldehyde and blocked with 10% serum for 1 h at RT; antigen retrieval was by heat mediation with a citrate buffer (pH6). Samples were incubated with primary antibody overnight at 4°C. A goat anti-rabbit IgG H&L (HRP) at 1/250 was used as secondary. Counter stained with Hematoxylin.