STAT1 Antibody (monoclonal, 12C7)

• Catalog Number: orb546999
• Category: Antibodies
• Description: STAT1 Antibody (monoclonal, 12C7)
• Species/Host: Mouse
• Clonality: Monoclonal
• Clone Number: 12C7
• Tested applications: FC, ICC, IF, IHC, WB
• Reactivity: Human, Monkey
• Isotype: Mouse IgG1
• Immunogen: E.coli-derived human STAT1 recombinant protein (Position: S2-A230). Human STAT1 shares 91.2% amino acid (aa) sequence identity with mouse STAT1.
• Concentration: Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.
• Dilution range: Western blot, 0.1-0.5μg/ml Immunohistochemistry (Paraffin-embedded Section), 0.5-1μg/ml Immunocytochemistry/Immunofluorescence, 2μg/ml Flow Cytometry, 1-3μg/1x106 cells
• Form/Appearance: Lyophilized
• Conjugation: Unconjugated
• MW: 91 kDa
• UniProt ID: P42224
• Storage: Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.
• Alternative names: Signal transducer and activator of transcription 1
• Note: For research use only
• Application notes: Add 0.2ml of distilled water will yield a concentration of 500μg/ml.
• Expiration Date: 12 months from date of receipt.

Flow Cytometry analysis of A431 cells using anti-STAT1 antibody (Blue line).Isotype control antibody (Green line) was mouse IgG .Unlabelled sample (Red line) was also used as a control.

WB analysis of STAT1 using anti-STAT1 antibody.Lane 1:human HeLa cell;2:human placenta tissue;3:human 22RV1 cell.4:monkey COS-7 cell;5:human SW620 cell;6:human HepG2 cell;7:human PANC-1 cell.

IHC analysis of STAT1 using anti STAT1 antibody. STAT1 was detected in paraffin-embedded section of human intestinal cancer tissue.

IHC analysis of STAT1 using anti STAT1 antibody. STAT1 was detected in paraffin-embedded section of human intestinal cancer tissue.

IHC analysis of STAT1 using anti STAT1 antibody. STAT1 was detected in paraffin-embedded section of human mammary cancer tissue.

IHC analysis of STAT1 using anti STAT1 antibody. STAT1 was detected in paraffin-embedded section of human tonsil tissue.