Src Antibody

• Catalog Number: orb182391
• Category: Antibodies
• Description: Src Antibody
• Species/Host: Rabbit
• Clonality: Polyclonal
• Tested applications: FC, IHC, WB
• Reactivity: Human
• Isotype: Rabbit IgG
• Immunogen: E.coli-derived human Src recombinant protein (Position: G2-D368). Human Src shares 97% and 99% amino acid (aa) sequences identity with mouse and rat Src, respectively.
• Concentration: Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.
• Dilution range: Western blot, 0.1-0.5μg/ml, Human Immunohistochemistry (Paraffin-embedded Section), 2-5μg/ml, Human, By Heat Flow Cytometry, 1-3 μg/1x106 cells, Human
• Form/Appearance: Lyophilized
• Conjugation: Unconjugated
• MW: 59835 MW
• UniProt ID: P12931
• Storage: Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.
• Alternative names: Proto-oncogene tyrosine-protein kinase Src;2.7.10.
• Note: For research use only
• Application notes: WB: The detection limit for Src is approximately 0.25ng/lane under reducing conditions. Tested Species: In-house tested species with positive results. By Heat: Boiling the paraffin sections in 10mM citrate buffer, pH6.0, for 20mins is required for the staining of formalin/paraffin sections. Other applications have not been tested. Optimal dilutions should be determined by end users. . Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
• Expiration Date: 12 months from date of receipt.

Flow Cytometry analysis of HepG2 cells using anti-Src antibody (Blue line).Isotype control antibody (Green line) was rabbit IgG. Unlabelled sample (Red line) was also used as a control.

WB analysis of Src using anti-Src antibody.Lane 1:human A549 cell;2:human RT4 cell;3:human PC-3 cell;4:human SH-SY5Y cell.

IHC analysis of Src using anti-Src antibody. Src was detected in a paraffin-embedded section of human liver cancer tissue.

IHC analysis of Src using anti-Src antibody. Src was detected in a paraffin-embedded section of human rectal cancer tissue.