SPHK1 Antibody

• Catalog Number: orb1271021
• Category: Antibodies
• Description: SPHK1 Antibody
• Species/Host: Rabbit
• Clonality: Polyclonal
• Tested applications: IHC-P, WB
• Reactivity: Human, Mouse, Rat
• Isotype: Rabbit Ig
• Immunogen: This SPHK1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 286-315 amino acids from the Central region of human SPHK1.
• Concentration: batch dependent
• Dilution range: For IHC-P starting dilution is: 1:25For WB starting dilution is: 1:1000
• Form/Appearance: Liquid
• Conjugation: Unconjugated
• MW: 43 kDa
• Target: SPHK1
• UniProt ID: Q9NYA1
• NCBI: Q9NYA1
• Storage: Store at 4°C for three months and -20°C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
• Buffer/Preservatives: Supplied in PBS with 0.09% (W/V) sodium azide.
• Alternative names: Sphingosine kinase 1, SK 1, SPK 1, SPHK1, SPHK, SP
• Note: For research use only
• Application notes: For IHC-P starting dilution is: 1:25For WB starting dilution is: 1:1000
• Expiration Date: 12 months from date of receipt.

Antibody staining SPHK1 in human heart tissue sections by Immunohistochemistry (IHC-P - paraformaldehyde-fixed, paraffin-embedded sections).

Antibody staining SPHK1 in human heart tissue sections by Immunohistochemistry (IHC-P - paraformaldehyde-fixed, paraffin-embedded sections).

Western Blot at 1:2000 dilution Lane 1: human liver lysate Lane 2: HUVEC whole cell lysate Lysates/proteins at 20 ug per lane.

Western Blot at 1:2000 dilution + human liver lysate Lysates/proteins at 20 ug per lane.

Western blot analysis of lysates from 293, HepG2, HUVEC, Raji, rat C6, mouse C2C12 cell line (from left to right), using SPHK1 Antibody at 1:1000 at each lane.

Antibody is used in Western blot to detect SPHK1 in mouse kidney tissue lysate.

Western blot analysis of anti-SPHK1 Antibody in 293 cell line lysates (35 ug/lane)