SP1 antibody

• Catalog Number: orb19050
• Category: Antibodies
• Description: Goat polyclonal antibody to SP1
• Species/Host: Goat
• Clonality: Polyclonal
• Tested applications: ELISA, WB
• Reactivity: Bovine, Canine, Human, Mouse, Porcine, Rat
• Dilution range: ELISA: 1:16000, WB: 0.3-1 μg/ml
• Conjugation: Unconjugated
• MW: 80.7; 79.9
• Target: SP1
• Entrez: 6667
• Protein Sequence: CSRIESPNENSNNSQ
• Storage: Aliquot and store at -20°C. Minimize freezing and thawing.
• Buffer/Preservatives: Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.
• Alternative names: anti SP1 antibody, anti Sp1 transcription factor a
• Note: For research use only
• Application notes: ELISA: Peptide ELISA: antibody detection limit dilution 1:16000.WB: Approx 100kDa band observed in Human HeLa lysates (calculated MW of 80.7kDa according to NP_612482.2). Recommended concentration: 0.3-1 μg/ml.
• Expiration Date: 12 months from date of receipt.

orb19050 staining (0.3ug/ml) of Hela lysate (RIPA buffer, 30ug total protein per lane). Primary incubated for 1 hour. Detected by western blot using chemiluminescence.