CHGA Antibody

• Catalog Number: orb1247992
• Category: Antibodies
• Description: CHGA Antibody
• Species/Host: Goat
• Clonality: Polyclonal
• Tested applications: ELISA, FC, IHC, WB
• Reactivity: Human
• Immunogen: The immunogen for this antibody is: C-QGAKERAHQQKKH
• Concentration: 500 ug/mL
• Dilution range: Peptide ELISA: antibody detection limit dilution 1:4000.Western Blot:Approx 50kDa band observed in Human Cerebellum and Kidney lysates. An additional band at 35kDa was observed in some lysates (calculated MW of 50.7 kDa according to NP_001266.1 isoform 1 and 34.3kDa according to NP_001288619.1 isoform 2). Recommended concentration: 1-2ug/ml. Primary incubation 1 hour at room temperature.Immunohistochemistry:Paraffin embedded Human Pancreas and Small Intestine. Recommended concentration: 3.75ug/ml.Flow Cytometry: Flow cytometric analysis of A549 cells. Recommended concentration: 10ug/ml.
• Form/Appearance: Liquid
• Conjugation: Unconjugated
• Target: CHGA
• UniProt ID: P10645
• NCBI: NP_001288619.1, NP_001266.1
• Storage: Aliquot and store at -20°C. Minimize freezing and thawing.
• Buffer/Preservatives: Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.
• Alternative names: chromogranin A precursor, chromogranin A (parathyr
• Note: For research use only
• Application notes: Peptide ELISA: antibody detection limit dilution 1:4000.Western Blot:Approx 50kDa band observed in Human Cerebellum and Kidney lysates. An additional band at 35kDa was observed in some lysates (calculated MW of 50.7 kDa according to NP_001266.1 isoform 1 and 34.3kDa according to NP_001288619.1 isoform 2). Recommended concentration: 1-2ug/ml. Primary incubation 1 hour at room temperature.Immunohistochemistry:Paraffin embedded Human Pancreas and Small Intestine. Recommended concentration: 3.75ug/ml.Flow Cytometry: Flow cytometric analysis of A549 cells. Recommended concentration: 10ug/ml.
• Expiration Date: 12 months from date of receipt.

orb1247992 (0.1 ug/ml) staining of isoform 1+ 2 Human Kidney lysate (35 ug protein in RIPA buffer). Detected by chemiluminescence.

orb1247992 (3.75 ug/ml) staining of paraffin embedded Human Pancreas. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.

orb1247992 (3.75 ug/ml) staining of paraffin embedded Human Small Intestine. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.

orb1247992 (2 ug/ml) staining of isoform 1 Human Brain (A) and isoform 1+2 (B) lysate (35 ug protein in RIPA buffer). Detected by chemiluminescence.

orb1247992 Flow cytometric analysis of paraformaldehyde fixed A549 cells (blue line), permeabilized with 0.5% Triton. Primary incubation 1hr (10 ug/ml) followed by Alexa Fluor 488 secondary antibody (1 ug/ml). IgG control: Unimmunized goat IgG (black line).