SP1 Antibody

• Catalog Number: orb1270161
• Category: Antibodies
• Description: SP1 Antibody
• Species/Host: Rabbit
• Clonality: Polyclonal
• Tested applications: FC, IF, IHC-P, WB
• Predicted Reactivity: Mouse, Rat
• Reactivity: Human
• Isotype: Rabbit Ig
• Immunogen: This SP1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 677-707 amino acids from the C-terminal region of human SP1.
• Concentration: batch dependent
• Dilution range: For WB starting dilution is: 1:500For IHC-P starting dilution is: 1:50~100For FACS starting dilution is: 1:10~50For IF starting dilution is: 1:10~50
• Form/Appearance: Liquid
• Conjugation: Unconjugated
• MW: 81 kDa
• Target: SP1
• UniProt ID: P08047
• NCBI: P08047
• Storage: Store at 4°C for three months and -20°C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
• Buffer/Preservatives: Supplied in PBS with 0.09% (W/V) sodium azide.
• Alternative names: Transcription factor Sp1, SP1, TSFP1
• Note: For research use only
• Application notes: For WB starting dilution is: 1:500For IHC-P starting dilution is: 1:50~100For FACS starting dilution is: 1:10~50For IF starting dilution is: 1:10~50
• Expiration Date: 12 months from date of receipt.

Flow cytometric analysis of K562 cells (right histogram) compared to a negative control cell (left histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.

Western Blot at 1:500 dilution + Hela whole cell lysate Lysates/proteins at 20 ug per lane.

Western blot analysis in K562 cell line lysates (35 ug/lane).

SP1 Antibody (C-term P692) immunohistochemistry analysis in formalin fixed and paraffin embedded human colon carcinoma followed by peroxidase conjugation of the secondary antibody and DAB staining.This data demonstrates the use o for immunohistochemistry.

Confocal immunofluorescent analysis with WiDr cell followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green). DAPI was used to stain the cell nuclear (blue).