SOD1 Antibody

• Catalog Number: orb1247848
• Category: Antibodies
• Description: SOD1 Antibody
• Species/Host: Goat
• Clonality: Polyclonal
• Tested applications: EIA, ELISA, WB
• Predicted Reactivity: Canine, Rat
• Reactivity: Human, Mouse
• Immunogen: The immunogen for this antibody is: C-SRKHGGPKDEERH
• Concentration: 500 ug/mL
• Dilution range: Peptide ELISA: antibody detection limit dilution 1:8000.Western Blot:Approx 18kDa band observed in Mouse Brain and Rat Spinal Cord lysates, and in lysates of cell line NIH3T3. Approx 20kDa observed in lysates of cell lines HEK293, HepG2 and MCF7 (calculated MW of 15.9kDa according to Human NP_000445.1, Mouse NP_035564.1 and Rat NP_058746.1). Recommended concentration: 0.01-0.03µg/ml. Primary incubation 1 hour at room temperature. Enzyme immunoassay: Sandwich-type ELISA with increasing amount of recombinant SOD1 captured by a rabbit antibody. Recommended reporter concentration: 1-2ug/ml
• Form/Appearance: Liquid
• Conjugation: Unconjugated
• Target: SOD1
• UniProt ID: P00441
• NCBI: NP_000445.1
• Storage: Aliquot and store at -20°C. Minimize freezing and thawing.
• Buffer/Preservatives: Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.
• Alternative names: SOD1, superoxide dismutase 1, soluble (amyotrophic
• Note: For research use only
• Application notes: Peptide ELISA: antibody detection limit dilution 1:8000.Western Blot:Approx 18kDa band observed in Mouse Brain and Rat Spinal Cord lysates, and in lysates of cell line NIH3T3. Approx 20kDa observed in lysates of cell lines HEK293, HepG2 and MCF7 (calculated MW of 15.9kDa according to Human NP_000445.1, Mouse NP_035564.1 and Rat NP_058746.1). Recommended concentration: 0.01-0.03µg/ml. Primary incubation 1 hour at room temperature. Enzyme immunoassay: Sandwich-type ELISA with increasing amount of recombinant SOD1 captured by a rabbit antibody. Recommended reporter concentration: 1-2ug/ml
• Expiration Date: 12 months from date of receipt.

orb1247848 (0.02 μg/ml) staining of Mouse Brain lysate (35 μg protein in RIPA buffer). Detected by chemiluminescence.

orb1247848 (0.01 μg/ml) staining of NIH3T3 (A), HEK293 (B), HepG2 (C) and MCF7 (D) lysates (35 μg protein in RIPA buffer). Detected by chemiluminescence.

orb1247848 (1.5 ug/ml) as the reporter antibody.