Superoxide Dismutase 1/SOD1 Antibody

• Catalog Number: orb251585
• Category: Antibodies
• Description: Superoxide Dismutase 1/SOD1 Antibody
• Species/Host: Rabbit
• Clonality: Polyclonal
• Tested applications: FC, ICC, IF, IHC, WB
• Reactivity: Human, Mouse, Rat
• Isotype: Rabbit IgG
• Immunogen: A synthetic peptide corresponding to a sequence at the C-terminus of Human SOD1 (116-146aa RTLVVHEKADDLGKGGNEESTKTGNAGSRLA), different from the related mouse and rat sequences by two amino acids.
• Concentration: Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.
• Dilution range: Western blot, 0.1-0.5μg/ml, Human, Mouse, Rat Immunohistochemistry(Paraffin-embedded Section), 0.5-1 μg/ml, Human, Mouse, Rat, By Heat Immunocytochemistry/Immunofluorescence, 2 μg/ml, Human Flow Cytometry, 1-3 μg/1x106 cells, Human
• Form/Appearance: Lyophilized
• Conjugation: Unconjugated
• MW: 15936 MW
• UniProt ID: P00441
• Storage: Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.
• Alternative names: Superoxide dismutase [Cu-Zn];1.15.1.1;Superoxide d
• Note: For research use only
• Application notes: WB: The detection limit for SOD1 is approximately 0.1ng/lane under reducing conditions. Tested Species: In-house tested species with positive results. Other applications have not been tested. Optimal dilutions should be determined by end users. . Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
• Expiration Date: 12 months from date of receipt.

Flow Cytometry analysis of A549 cells using anti-SOD1 antibody (Blue line).Isotype control antibody (Green line) was rabbit IgG .Unlabelled sample (Red line) was also used as a control.

WB analysis of SOD1 using anti-SOD1 antibody.Lane 1:human HeLa cell;2:human Jurkat cell;3:rat brain tissue;4:rat spleen tissue;5:mouse brain tissue;6:mouse spleen tissue.

IF analysis of SOD1 using anti-SOD1 antibody.SOD1 was detected in immunocytochemical section of Hep2 cells.

IHC analysis of SOD1 using anti-SOD1 antibody.SOD1 was detected in paraffin-embedded section of human intestinal cancer tissue.

IHC analysis of SOD1 using anti-SOD1 antibody.SOD1 was detected in paraffin-embedded section of human intestinal cancer tissue.

IHC analysis of SOD1 using anti-SOD1 antibody.SOD1 was detected in paraffin-embedded section of human lung cancer tissue.

IHC analysis of SOD1 using anti-SOD1 antibody.SOD1 was detected in paraffin-embedded section of human lung cancer tissue.

IHC analysis of SOD1 using anti-SOD1 antibody.SOD1 was detected in paraffin-embedded section of human mammary cancer tissue.

IHC analysis of SOD1 using anti-SOD1 antibody.SOD1 was detected in paraffin-embedded section of human mammary cancer tissue.

IHC analysis of SOD1 using anti-SOD1 antibody.SOD1 was detected in paraffin-embedded section of rat liver tissue.

IHC analysis of SOD1 using anti-SOD1 antibody.SOD1 was detected in paraffin-embedded section of mouse liver tissue.