SOD1 antibody

• Catalog Number: orb19518
• Category: Antibodies
• Description: Goat polyclonal antibody to SOD1
• Species/Host: Goat
• Clonality: Polyclonal
• Tested applications: ELISA, IF, WB
• Reactivity: Canine, Human, Mouse, Rat
• Dilution range: ELISA: 1:8000, WB: 0.01-0.03 μg/ml, IHC-P: 10 μg/ml
• Conjugation: Unconjugated
• MW: 15.9
• Target: SOD1
• Entrez: 6647
• Protein Sequence: SRKHGGPKDEERH
• Storage: Aliquot and store at -20°C. Minimize freezing and thawing.
• Buffer/Preservatives: Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.
• Alternative names: anti SOD1 antibody, anti superoxide dismutase 1, s
• Note: For research use only
• Application notes: ELISA: Peptide ELISA: antibody detection limit dilution 1:8000.WB: Approx 18kDa band observed in Mouse Brain and Rat Spinal Cord lysates (calculated MW of 15.9kDa according to Human NP_000445.1, Mouse NP_035564.1 and Rat NP_058746.1). Recommended concentration: 0.01-0.03 μg/ml.
• Expiration Date: 12 months from date of receipt.

orb19518 (0.02ug/ml) staining of Mouse Brain lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.

Western blot analysis of NIH3T3 (Lane 1), HEK293 (Lane 2), HepG2 (Lane 3) and MCF7 (Lane 4) lysates using SOD1 antibody

Line graph illustrates about the Ag-Ab reactions using different concentrations of antigen and serial dilutions of using SOD1 antibody