SMN antibody

• Catalog Number: orb76815
• Category: Antibodies
• Description: Mouse monoclonal antibody to SMN
• Species/Host: Mouse
• Clonality: Monoclonal
• Clone Number: 2B1
• Tested applications: ELISA, FC, ICC, IHC-P, IP, WB
• Reactivity: Human, Mouse, Xenopus
• Isotype: IgG1
• Immunogen: Purified recombinant His6 tagged-SMN protein (human)
• Concentration: 1mg/ml
• Dilution range: Optimal antibody dilution should be determined by titration, however as a guideline try at; IHC-P: Use a concentration of 5 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol WB: 1µg/ml staining in HeLa (0.1µg/ml) staining in HepG2. Detects a band of approximately 42 kDa (predicted molecular weight: 35 kDa) ICC/IF: 1/100 on paraformaldehyde fixed HeLa cells, permeabilized with 0.15% Triton.
• Conjugation: Unconjugated
• Target: Gemin 1
• Entrez: 6607, 20595, 6606, 3187, 3185
• UniProt ID: p31943, p52597, q16637, p97801
• Buffer/Preservatives: Purified antibody (from supernatant) containing PBS + 0.1% sodium az
• Alternative names: BCD541 antibody;Gemin 1 antibody;Gemin-1 antibody;
• Note: For research use only
• Application notes: A/J Sp2/0 cell line
• Expiration Date: 12 months from date of receipt.

Confocal immunofluorescence analysis of paraffin-embedded A/J & Sp2/0 cell line using SMN antibody