STMN1 Antibody

• Catalog Number: orb1239686
• Category: Antibodies
• Description: STMN1 Antibody
• Species/Host: Rabbit
• Clonality: Polyclonal
• Tested applications: ELISA, IHC-P, WB
• Predicted Reactivity: Bovine, Gallus, Porcine
• Reactivity: Human, Mouse, Rat
• Isotype: IgG
• Immunogen: Anti-op18 antibody (orb1239686) was raised against a peptide corresponding to 20 amino acids near the carboxy terminus of human op18. The immunogen is located within the last 50 amino acids of op18.
• Concentration: 1 mg/mL
• Dilution range: WB: 0.5-2 μg /mL; IHC: 2.5 μg /mL; IF: 20 μg /mL.Antibody validated: Western Blot in human, mouse and rat samples; Immunohistochemistry in human samples. All other applications and species not yet tested.
• Form/Appearance: Liquid
• Conjugation: Unconjugated
• MW: Predicted: 17kDObserved: 17 kD
• Target: STMN1
• UniProt ID: P16949
• NCBI: AAH82228
• Storage: op18 antibody can be stored at 4°C for three months and -20°C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
• Buffer/Preservatives: op18 Antibody is supplied in PBS containing 0.02% sodium azide.
• Alternative names: op18 Antibody: Lag, SMN, OP18, PP17, PP19, PR22, L
• Note: For research use only
• Expiration Date: 12 months from date of receipt.

Western Blot Validation in Mouse EL4 Cell Lines. Loading: 15 µg of lysates per lane. Antibodies: op18 orb1239686 (A: 0.5 µg/mL, B: 1 µg /mL, C: 2 µg /mL), 1h incubation at RT in 5% NFDM/TBST. Secondary: Goat anti-rabbit IgG HRP conjugate at 1:10000 dilution.

Independent Antibody Validation (IAV) via Protein Expression Profile in Cell Lines. Loading: 15 µg of lysates per lane. Antibodies: op18 orb1239686 (2 µg/mL), op18 orb1239690 (2 µg /mL), and GAPDH orb1239765 (0.02 µg /mL), 1h incubation at RT in 5% NFDM/TBST. Secondary: Goat anti-rabbit IgG HRP conjugate at 1:10000 dilution.

Western Blot Validation in Human Cell Lines. Loading: 15 µg of cell lysate per lane. Antibodies: op18 orb1239686 (1 µg /mL), 1h incubation at RT in 5% NFDM/TBST. Secondary: Goat anti-rabbit IgG HRP conjugate at 1:10000 dilution.

Western Blot Validation in Mouse and Rat Cell Lines. Loading: 15 µg of lysates per lane. Antibodies: op18 orb1239686 (1µg /mL), 1h incubation at RT in 5% NFDM/TBST. Secondary: Goat anti-rabbit IgG HRP conjugate at 1:10000 dilution.

Immunohistochemistry Validation of op18 in Human Brain Tissue. Immunohistochemical analysis of paraffin-embedded Human Brain Tissue using anti-op18 antibody (orb1239686) at 2.5 µg /ml. Tissue was fixed with formaldehyde and blocked with 10% serum for 1 h at RT; antigen retrieval was by heat mediation with a citrate buffer (pH6). Samples were incubated with primary antibody overnight at 4°C. A goat anti-rabbit IgG H&L (HRP) at 1/250 was used as secondary. Counter stained with Hematoxylin.