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Catalog Number | orb316609 |
---|---|
Category | Antibodies |
Description | SMC3 Antibody |
Species/Host | Rabbit |
Clonality | Polyclonal |
Tested applications | IHC, WB |
Predicted Reactivity | Bovine, Equine, Gallus, Monkey, Rabbit |
Reactivity | Human, Mouse, Rat |
Isotype | Rabbit IgG |
Immunogen | A synthetic peptide corresponding to a sequence at the C-terminus of human SMC3 (1178-1216aa ELLESADKFYGVKFRNKVSHIDVITAEMAKDFVEDDTTH), identical to the related mouse sequence. |
Concentration | Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml. |
Dilution range | Immunohistochemistry (Paraffin-embedded Section), 0.5-1μg/ml, Human, Mouse, Rat, By Heat Western blot, 0.1-0.5μg/ml, Human, Mouse, Rat |
Form/Appearance | Lyophilized |
Conjugation | Unconjugated |
MW | 141542 MW |
UniProt ID | Q9UQE7 |
Storage | Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles. |
Alternative names | Structural maintenance of chromosomes protein 3;SM Read more... |
Note | For research use only |
Application notes | Tested Species: In-house tested species with positive results. By Heat: Boiling the paraffin sections in 10mM citrate buffer, pH6.0, for 20mins is required for the staining of formalin/paraffin sections. Other applications have not been tested. Optimal dilutions should be determined by end users. . Add 0.2ml of distilled water will yield a concentration of 500ug/ml. |
Expiration Date | 12 months from date of receipt. |
WB analysis of SMC3 using anti-SMC3 antibody.Lane 1:Rat Brain Tissue;2:Rat Liver Tissue;3:Rat Testis Tissue;4:HELA Cell;5:A549 Cell;6:MCF-7 Cell;7:NIH3T3 Cell.
IHC analysis of SMC3 using anti-SMC3 antibody. SMC3 was detected in a paraffin-embedded section of human intestinal cancer tissue.
IHC analysis of SMC3 using anti-SMC3 antibody. SMC3 was detected in a paraffin-embedded section of mouse intestine tissue.
IHC analysis of SMC3 using anti-SMC3 antibody. SMC3 was detected in a paraffin-embedded section of rat kidney tissue.
IHC, WB | |
Human, Mouse, Rat | |
Mouse | |
Monoclonal | |
Unconjugated |
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