SMC3 Antibody

• Catalog Number: orb1530742
• Category: Antibodies
• Description: SMC3 Antibody
• Species/Host: Rabbit
• Clonality: Polyclonal
• Tested applications: IHC, IP, WB
• Reactivity: Human, Mouse
• Immunogen: between 1175 and C-term
• Concentration: 1000 µg/ml
• Dilution range: WB - 1:10,000 - 1:25,000; IP - 2 - 10 µg/mg lysate; IHC - 1:1,000 to 1:5,000. Epitope retrieval with citrate buffer pH6.0 is recommended for FFPE tissue sections.
• Form/Appearance: Whole IgG
• Conjugation: Unconjugated
• Target: SMC3
• UniProt ID: Q9UQE7
• NCBI: NP_005436.1
• Storage: 2 - 8°C
• Buffer/Preservatives: Tris-citrate/phosphate buffer, pH 7 to 8 containing 0.09% Sodium Azide
• Alternative names: BAM; CSPG6; structural maintenance of chromosomes
• Note: For research use only
• Application notes: Format: Whole IgG
• Expiration Date: 12 months from date of receipt.

Detection of mouse SMC3 by western blot.Samples: Whole cell lysate from NIH 3T3, CT26, CH27, TCMK-1, and BW5147.3 cells. Antibody: Affinity purified rabbit anti-SMC3 antibody at 0.04 µg/ml.

Detection of human SMC3 by western blot.Samples: Whole cell lysate from HeLa, Jurkat, LNCaP, U2OS, and HEK293T cells. Antibody: Affinity purified rabbit anti-SMC3 antibody at 0.04 µg/ml.

Detection of mouse SMC3 by immunohistochemistry. Sample: FFPE section of mouse renal cell carcinoma. Antibody: Affinity purified rabbit anti-SMC3 used at 1:5, 000 (0.2µg/ml).

Detection of human SMC3 by immunohistochemistry. Sample: FFPE section of human ovarian carcinoma. Antibody: Affinity purified rabbit anti-SMC3 used at 1:5, 000 (0.2µg/ml).

Detection of human SMC3 by western blot of immunoprecipitates.Samples: Whole cell lysate from HEK293T cells. Antibodies: Affinity purified rabbit anti-SMC3 antibody.