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Catalog Number | orb19242 |
---|---|
Category | Antibodies |
Description | Goat polyclonal antibody to SH2D1A |
Species/Host | Goat |
Clonality | Polyclonal |
Tested applications | ELISA, IP, WB |
Reactivity | Human, Porcine |
Dilution range | ELISA: 1:32000, WB: 0.2-0.6 μg/ml |
Conjugation | Unconjugated |
MW | 14.2; 13.9 |
Target | SH2D1A/SLAM associated protein |
Entrez | 4068 |
Protein Sequence | QYPVEKKSSARSTQ |
Storage | Aliquot and store at -20°C. Minimize freezing and thawing. |
Buffer/Preservatives | Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing. |
Alternative names | anti SH2D1A antibody, anti SH2 domain protein 1A, Read more... |
Note | For research use only |
Application notes | ELISA: Peptide ELISA: antibody detection limit dilution 1:32000.WB: Approx 16kDa band observed in Human Lymph Node lysates (calculated MW of 14.2kDa according to NP_002342.1). In transfected HEK293 transiently expressing SH2D1A a band of approx. 17kDa is observed. This band is not observed in the non-transfected HEK293. Recommended concentration: 0.2-0.6 μg/ml. |
Expiration Date | 12 months from date of receipt. |
orb19242 (0.2ug/ml) staining of human lymph node lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
orb19242 overexpressing SH2D1A (RC204723) and probed with orb19242 (mock transfection in first lane), tested by Origene.
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