SH2D1A Antibody

• Catalog Number: orb1246957
• Category: Antibodies
• Description: SH2D1A Antibody
• Species/Host: Goat
• Clonality: Polyclonal
• Tested applications: ELISA, IP, WB
• Predicted Reactivity: Porcine
• Reactivity: Human
• Immunogen: The immunogen for this antibody is: C-QYPVEKKSSARSTQ
• Antibody Type: Primary Antibody
• Concentration: 500 ug/mL
• Form/Appearance: Liquid
• Conjugation: Unconjugated
• Target: SH2D1A
• UniProt ID: O60880
• NCBI: NP_001108409.1, NP_002342.1
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Buffer/Preservatives: Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.
• Alternative names: SH2D1A, SH2 domain protein 1A, Duncan's disease (l
• Note: For research use only
• Application notes: Peptide ELISA: antibody detection limit dilution 1:32000.Western Blot:Approx 16kDa band observed in Human Lymph Node lysates (calculated MW of 14.2kDa according to NP_002342.1). In transfected HEK293 transiently expressing SH2D1A a band of approx. 17kDa is observed. This band is not observed in the non-transfected HEK293. Recommended concentration: 0.2-0.6ug/ml.Immunoprecipitation:This antibody was deemed fit for IP under native conditions (observations from anonymous customer).
• Expiration Date: 12 months from date of receipt.

orb1246957 (0.2 ug/ml) staining of human lymph node lysate (35 ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.

HEK293 overexpressing SH2D1A and probed with orb1246957 (mock transfection in first lane).