SH2D1A Antibody

• Catalog Number: orb1246958
• Category: Antibodies
• Description: SH2D1A Antibody
• Species/Host: Goat
• Clonality: Polyclonal
• Tested applications: ELISA, IP, WB
• Predicted Reactivity: Mouse, Rat
• Reactivity: Human
• Immunogen: The immunogen for this antibody is: C-HKRYFRKIKN
• Concentration: 500 ug/mL
• Dilution range: Peptide ELISA: antibody detection limit dilution 1:16000.Western Blot:Approx 16kDa band observed in Human Thymus lysates (calculated MW of 14.2xkDa according to NP_002342.1). In transfected HEK293 transiently expressing SH2D1A a band of approx. 16kDa is observed. This band is not observed in the non-transfected HEK293. Recommended concentration: 0.3-1ug/ml.Immunoprecipitation:This antibody was deemed fit for IP under native conditions (observations from anonymous customer).
• Form/Appearance: Liquid
• Conjugation: Unconjugated
• Target: SH2D1A
• UniProt ID: O60880
• NCBI: NP_001108409.1, NP_002342.1
• Storage: Aliquot and store at -20°C. Minimize freezing and thawing.
• Buffer/Preservatives: Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.
• Alternative names: SH2D1A, SAP, SH2 domain protein 1A, Duncan's disea
• Note: For research use only
• Application notes: Peptide ELISA: antibody detection limit dilution 1:16000.Western Blot:Approx 16kDa band observed in Human Thymus lysates (calculated MW of 14.2xkDa according to NP_002342.1). In transfected HEK293 transiently expressing SH2D1A a band of approx. 16kDa is observed. This band is not observed in the non-transfected HEK293. Recommended concentration: 0.3-1ug/ml.Immunoprecipitation:This antibody was deemed fit for IP under native conditions (observations from anonymous customer).
• Expiration Date: 12 months from date of receipt.

orb1246958 staining (0.3 ug/ml) of Human Thymus lysate (RIPA buffer, 30 ug total protein per lane). Primary incubated for 1 hour. Detected by western blot using chemiluminescence.

HEK293 overexpressing SH2D1A and probed with orb1246958 (mock transfection in first lane).