SET Antibody

• Catalog Number: orb1526942
• Category: Antibodies
• Description: SET Antibody
• Species/Host: Rabbit
• Clonality: Polyclonal
• Tested applications: IHC, IP, WB
• Reactivity: Human, Mouse
• Immunogen: between 125 and 175
• Concentration: 200 µg/ml
• Dilution range: WB - 1:2,000 - 1:10,000; IP - 2 - 10 µg/mg lysate; IHC - 1:500 - 1:2,000. Epitope retrieval with citrate buffer pH 6.0 is recommended for FFPE tissue sections.
• Form/Appearance: Whole IgG
• Conjugation: Unconjugated
• Target: SET
• UniProt ID: Q01105
• NCBI: NP_001116293.1
• Storage: 2 - 8°C
• Buffer/Preservatives: Tris-buffered Saline containing 0.1% rAlbumin and 0.09% Sodium Azide
• Alternative names: I2PP2A; protein phosphatase type 2A inhibitor; pro
• Note: For research use only
• Application notes: Format: Whole IgG
• Expiration Date: 12 months from date of receipt.

Detection of mouse SET by western blot. Samples: Whole cell lysate (10 µg) from NIH 3T3, CT26, CH27, TCMK-1, and BW5147.3 cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-SET antibody orb1526942 used for WB at 0.04 µg/ml. Detection: Chemiluminescence with an exposure time of 75 seconds.

Detection of human SET by western blot. Samples: Whole cell lysate (10 µg) from Hep-G2, HeLa, Jurkat, MCF-7, and HEK293T cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-SET antibody orb1526942 used for WB at 0.04 µg/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.

Detection of human SET by western blot of immunoprecipitates. Samples: Whole cell lysate (1.0 mg per IP reaction; 20% of IP loaded) from HEK293T cells prepared using NETN lysis buffer. Antibodies: Affinity purified rabbit anti-SET antibody orb1526942 used for IP at 6 µg per reaction.

Detection of mouse SET by immunohistochemistry.Sample: FFPE section of mouse plasmacytoma. Antibody: Affinity purified rabbit anti-SET (Cat. No. orb1526942) used at a dilution of 1: 1, 000 (0.2µg/ml). Detection: DAB

Detection of human SET by immunohistochemistry.Sample: FFPE section of human ovarian carcinoma. Antibody: Affinity purified rabbit anti-SET (Cat. No. orb1526942) used at a dilution of 1: 1, 000 (0.2µg/ml). Detection: DAB