VSIR Antibody

• Catalog Number: orb1240295
• Category: Antibodies
• Description: VSIR Antibody
• Species/Host: Mouse
• Clonality: Monoclonal
• Clone Number: 8E11
• Tested applications: ELISA, FC, ICC, IF, IHC-P
• Reactivity: Human
• Isotype: IgG1
• Immunogen: VISTA antibody was raised against the extracellular domain of human VISTA.
• Concentration: 1 mg/mL
• Dilution range: VISTA antibody can be used for immunohistochemistry starting at 2 μg/mL and immunocytochemistry starting at 1 μg/mL. For immunofluorescence start at 2 μg/mL. Flow cytometry at 10 μg/ml.Antibody validated: Immunohistochemistry in human samples; Immunocytochemistry in human samples; Immunofluorescence in human samples and Flow Cytometry in mouse samples. All other applications and species not yet tested.
• Form/Appearance: Liquid
• Conjugation: Unconjugated
• MW: Predicted: 31 kDa Observed: 47 kDa
• Target: VSIR
• UniProt ID: Q9H7M9
• NCBI: NP_071436
• Storage: VISTA antibody can be stored at 4°C for three months and -20°C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
• Buffer/Preservatives: VISTA Antibody is supplied in PBS containing 0.02% sodium azide and 50% glycerol.
• Alternative names: VISTA Antibody: VISTA molecule, VSIR, B7-H5, B7H5,
• Note: For research use only
• Application notes: VISTA antibody can be used for immunohistochemistry starting at 2 μg/mL and immunocytochemistry starting at 1 μg/mL. For immunofluorescence start at 2 μg/mL. Flow cytometry at 10 μg/ml.Antibody validated: Immunohistochemistry in human samples; Immunocytochemistry in human samples; Immunofluorescence in human samples and Flow Cytometry in mouse samples. All other applications and species not yet tested.
• Expiration Date: 12 months from date of receipt.

Immunocytochemistry of VISTA in transfected HEK293 cells with VISTA antibody at 1 μg/mL. Lower left: Immunocytochemistry in transfected HEK293 cells with control mouse IgG antibody at 1 μg/mL.

Immunofluorescence of VISTA in transfected HEK293 cells with VISTA antibody at 2 μg/mL. Green: VISTA Antibody [8E11] (orb1240295) Blue: DAPI staining

Immunofluorescence of VISTA in human lymphoma tissue with VISTA antibody at 10 μg/mL. Red: VISTA Antibody [8E11] (orb1240295) Blue: DAPI staining

Immunofluorescence of VISTA in human spleen tissue with VISTA antibody at 10 μg/mL. Red: VISTA Antibody [8E11] (orb1240295) Blue: DAPI staining

Immunohistochemistry of VISTA in human lymphoma tissue with VISTA antibody at 2 μg/mL.

Flow cytometry analysis of VISTA overexpressing HEK293 cells using VISTA antibody and control mouse IgG antibody at 10 μg/ml. Blue: Untransfected HEK293 cells. Yellow: VISTA overexpressing HEK293 cells.

Titration curve analysis of VISTA antibody to detect recombinant VISTA in ELISA at decreasing concentrations.