SEMA4D Antibody

• Catalog Number: orb1523072
• Category: Antibodies
• Description: SEMA4D Antibody
• Species/Host: Rabbit
• Clonality: Polyclonal
• Tested applications: IP, WB
• Reactivity: Human
• Immunogen: Between 812 and 862
• Concentration: 1000 µg/ml
• Dilution range: WB - 1:2,000 - 1:10,000; IP - 2 - 10 µg/mg lysate
• Form/Appearance: Whole IgG
• Conjugation: Unconjugated
• Target: SEMA4D
• UniProt ID: Q92854
• NCBI: NP_006369.3
• Storage: 2 - 8°C
• Buffer/Preservatives: Tris-citrate/phosphate buffer, pH 7 to 8 containing 0.09% Sodium Azide
• Alternative names: C9orf164; semaphorin-4D; SEMAJ; sema domain, immun
• Note: For research use only
• Application notes: Format: Whole IgG
• Expiration Date: 12 months from date of receipt.

Detection of human SEMA4D by western blot. Samples: Whole cell lysate (50 µg) prepared using NETN or RIPA buffer from HeLa, HEK293T, and Jurkat cells. Antibodies: Affinity purified rabbit anti-SEMA4D antibody orb1523072 used for WB at 0.1 µg/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.

Detection of human SEMA4D by western blot of immunoprecipitates. Samples: Whole cell lysate (0.5 or 1.0 mg per IP reaction; 20% of IP loaded) prepared using RIPA buffer from Jurkat cells. Antibodies: Affinity purified rabbit anti-SEMA4D antibody orb1523072 used for IP at 6 µg per reaction.