CD100 Antibody (FITC)

• Catalog Number: orb1570908
• Category: Antibodies
• Description: CD100, also known as semaphorin 4D, is a homodimerizing type I transmembrane glycoprotein containing an extracellular semaphorin domain. It is expressed on most hematopoietic cells with the exception of immature bone marrow cells, erythrocytes and platelets. A 120 kDa soluble form is generated from the transmembrane form by proteolytic cascade following primary T and B cell activation. It seems CD100 acts through dampening CD72-mediated negative signaling. CD100 promotes angiogenesis, invasive growth, proliferation and anti-apoptosis of cancer cells in vitro. Higher expression levels of CD100 correlate with poor survival in soft tissue sarcoma patients.
• Clonality: Monoclonal
• Clone Number: 133-1C6
• Tested applications: FC
• Reactivity: Human
• Isotype: Mouse IgM
• Immunogen: PHA stimulated human PBL
• Conjugation: FITC
• Target: CD100
• Entrez: 10507
• UniProt ID: Q92854
• Storage: Store at 2-8°C. Protect from prolonged exposure to light. Do not freeze.
• Buffer/Preservatives: Stabilizing Tris buffered saline (TBS), pH 8.0, 15 mM sodium azide
• Alternative names: COLL4, SEMA4D, semaphorin 4D
• Note: For research use only
• Application notes: Flow cytometry: The reagent is designed for analysis of human blood cells using 4 μl reagent / 100 μl of whole blood or 106 cells in a suspension. The content of a vial (0.4 ml) is sufficient for 100 tests.
• Expiration Date: 12 months from date of receipt.

Flow cytometry multicolor surface staining of human lymphocytes stained using anti-human CD100 (133-1C6) FITC antibody (4 μl reagent / 100 μl of peripheral whole blood) and anti-human CD56 (LT56) APC antibody (10 μl reagent / 100 μl of peripheral whole blood).

Separation of human CD100 positive CD56 positive lymphocytes (red-filled) from CD100 negative CD56 negative lymphocytes (black-dashed) in flow cytometry analysis (surface staining) of human peripheral whole blood stained using anti-human CD100 (133-1C6) FITC antibody (4 μl reagent / 100 μl of peripheral whole blood).

Flow cytometry surface staining pattern of human peripheral whole blood stained using anti-human CD100 (133-1C6) FITC antibody (4 μl reagent / 100 μl of peripheral whole blood).