SCF Rabbit Polyclonal Antibody (FITC)

• Catalog Number: orb16292
• Category: Antibodies
• Description: SCF Rabbit Polyclonal Antibody (FITC)
• Target: KITLG
• Clonality: Polyclonal
• Species/Host: Rabbit
• Isotype: IgG
• Conjugation: FITC
• Reactivity: Human, Mouse, Rat
• Predicted Reactivity: Goat
• Concentration: 1mg/ml
• Buffer/Preservatives: 0.01M TBS (pH7.4) with 1% rAlbumin, 0.02% Proclin300 and 50% Glycerol.
• Purification: Affinity purified by Protein A
• Immunogen: KLH conjugated synthetic peptide derived from human SCF
• RRID: AB_10747720
• Tested applications: FC, IF
• Dilution range: Flow-Cyt=1ug/Test, IF=1:100-500
• Antibody Type: Primary Antibody
• Storage: Shipped at 4°C. Store at -20°C for one year. Avoid repeated freeze/thaw cycles.
• Alternative names: Stem Cell Factor; C kit ligand; Ckit ligand; DKFZp
• Research Area: Colony Stimulating Factors (CSF), Immunology, Mole
• Note: For research use only
• Expiration Date: 12 months from date of receipt.

Blank control (Black line): U-87MG (Black). Primary Antibody (green line): Rabbit Anti-SCF antibody (orb16292), dilution: 3 µg/10^6 cells, Isotype Control Antibody (orange line): Rabbit IgG-FITC. Protocol The cells were fixed with 4% PFA (10 min at room temperature) and then permeabilized with PBST for 20 min at room temperature. The cells were then incubated in 5% BSA to block non-specific protein-protein interactions for 30 min at room temperature. Cells stained with Primary Antibody for 30 min at room temperature. Acquisition of 20000 events was performed.