SCF Rabbit Polyclonal Antibody (BF647)

• Catalog Number: orb1586496
• Category: Antibodies
• Description: SCF Rabbit Polyclonal Antibody (BF647)
• Target: KITLG
• Clonality: Polyclonal
• Species/Host: Rabbit
• Isotype: IgG
• Conjugation: BF647
• Reactivity: Human, Mouse, Rat
• Predicted Reactivity: Goat
• Concentration: 1mg/ml
• Buffer/Preservatives: 0.01M TBS (pH7.4) with 1% rAlbumin, 0.02% Proclin300 and 50% Glycerol.
• Immunogen: KLH conjugated synthetic peptide derived from human SCF
• Tested applications: FC, IF
• Dilution range: Flow-Cyt=1ug/Test, IF=1:100-500
• Antibody Type: Primary Antibody
• Storage: Shipped at 4°C. Store at -20°C for one year. Avoid repeated freeze/thaw cycles.
• Alternative names: Stem Cell Factor; C kit ligand; Ckit ligand; DKFZp
• Note: For research use only

Blank control: Mouse spleen. Primary Antibody (green line): Rabbit Anti-SCF antibody (orb1586496), dilution: 2 µg/10^6 cells, Isotype Control Antibody (orange line): Rabbit IgG. Protocol, The cells were fixed with 4% PFA (10 min at room temperature) and then permeabilized with PBST for 20 min at room temperature. The cells were then incubated in 5% BSA to block non-specific protein-protein interactions for 30 min at at room temperature. Cells stained with Primary Antibody for 30 min at room temperature. Acquisition of 20000 events was performed.