S100A8 Antibody

• Catalog Number: orb2637486
• Category: Antibodies
• Description: The S100 family of proteins are found in vertebrates and are characterized by two calcium-binding sites that have a helix-loop-helix conformation. The family of 24 is divided into three subgroups based on function: those with intracellular, intracellular plus extracellular, and extracellular regulatory effects. The calcium-binding protein S100A8, also called MRP8 or Myeloid-related protein 8, and Calgranulin A, forms a heterodimer with S100A9, called Calprotectin. The heterodimer is expressed by granulocytes, monocytes, and early differentiation states of macrophages, and is known to be an important pro-inflammatory mediator in acute and chronic inflammation. S100A8 and 9 are DAMPs, or Damage-associated molecular pattern molecules, and are able to activate receptors of the innate immune system, one being TLR4.
• Species/Host: Mouse
• Clonality: Monoclonal
• Clone Number: SA6279
• Tested applications: WB
• Reactivity: Human
• Isotype: Mouse IgG1, kappa
• Immunogen: A recombinant protein fragment from the N-terminal region of human S100A8 was used as the immunogen for this antibody.
• Antibody Type: Primary Antibody
• Dilution range: Western blot: 2-4ug/ml
• Purity: Protein G affinity chromatography
• Conjugation: Unconjugated
• Formula: 0.5 mg/ml with 0.1 mg/ml BSA (US sourced), 0.05% sodium azide
• Hazard Information: This S100A8 antibody is available for research use only.
• Entrez: 6279
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Buffer/Preservatives: 1X PBS, pH 7.4
• Note: For research use only
• Application notes: Provided assay concentrations are suggestions only, S100A8 antibody titration may be required for optimal results.
• Expiration Date: 12 months from date of receipt.

Western blot testing of human samples with S100A8 antibody (clone SA6279) at 2 ug/ml. Predicted molecular weight ~11 kDa.