S100A6 Antibody

• Catalog Number: orb1274455
• Category: Antibodies
• Description: S100A6 Antibody
• Species/Host: Rabbit
• Clonality: Polyclonal
• Tested applications: WB
• Reactivity: Human, Mouse
• Isotype: IgG
• Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 1-90 of human S100A6 (NP_055439.1).
• Concentration: batch dependent
• Dilution range: WB: 1:200 - 1:2000
• Form/Appearance: Liquid
• Conjugation: Unconjugated
• MW: Observed: 10kDa
• Target: S100A6
• UniProt ID: P06703
• Storage: Store at -20°C. Avoid freeze / thaw cycles.
• Buffer/Preservatives: PBS with 0.02% sodium azide, 50% glycerol, pH 7.3.
• Alternative names: S100A6, 2A9, 5B10, CABP, CACY, PRA, MLN 4, Protein
• Note: For research use only
• Application notes: WB: 1:200 - 1:2000
• Expiration Date: 12 months from date of receipt.

Western blot analysis of extracts of various cell lines, using S100A6 antibody (orb1274455) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25 ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 10s.

Western blot analysis of extracts from normal (control) and S100A6 knockout (KO) HeLa cells, using S100A6 antibody (orb1274455) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25 ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 60s.