S100 alpha 6/S100A6 Antibody

• Catalog Number: orb315184
• Category: Antibodies
• Description: S100 alpha 6/S100A6 Antibody
• Species/Host: Rabbit
• Clonality: Polyclonal
• Tested applications: FC, ICC, IF, IHC, WB
• Reactivity: Human, Mouse, Rat
• Isotype: Rabbit IgG
• Immunogen: E.coli-derived human S100 alpha 6 recombinant protein (Position: M1-G90). Human S100 alpha 6 shares 96.6% and 95.5% amino acid (aa) sequence identity with mouse and rat S100 alpha 6, respectively.
• Concentration: Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.
• Dilution range: Western blot, 0.1-0.5μg/ml, Human, Rat Immunohistochemistry (Paraffin-embedded Section), 0.5-1μg/ml, Human, Mouse, Rat, By Heat Immunocytochemistry/Immunofluorescence, 5μg/ml, Human Flow Cytometry, 1-3μg/1x106 cells, Human
• Form/Appearance: Lyophilized
• Conjugation: Unconjugated
• MW: 10180 MW
• UniProt ID: P06703
• Storage: Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.
• Alternative names: Protein S100-A6;Calcyclin;Growth factor-inducible
• Note: For research use only
• Application notes: Tested Species: In-house tested species with positive results. By Heat: Boiling the paraffin sections in 10mM citrate buffer, pH6.0, for 20mins is required for the staining of formalin/paraffin sections. Other applications have not been tested. Optimal dilutions should be determined by end users. . Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
• Expiration Date: 12 months from date of receipt.

Flow Cytometry analysis of SiHa cells using anti-S100A6 antibody (Blue line).Isotype control antibody (Green line) was rabbit IgG .Unlabelled sample (Red line) was also used as a control.

Flow Cytometry analysis of A431 cells using anti-S100A6 antibody (Blue line).Isotype control antibody (Green line) was rabbit IgG .Unlabelled sample (Red line) was also used as a control.

WB analysis of S100A6 using anti-S100A6 antibody.Lane 1:human HeLa cell;2:human SW620 cell;3:human placenta tissue;4:mouse NIH/3T3 cell.

IF analysis of S100A6 using anti-S100A6 antibody.S100A6 was detected in an immunocytochemical section of A431 cells.

IHC analysis of S100A6 using anti-S100A6 antibody.S100A6 was detected in paraffin-embedded section of Human Placenta tissues.

IHC analysis of S100A6 using anti-S100A6 antibody.S100A6 was detected in paraffin-embedded section of Human Mammary Cancer tissues.

IHC analysis of S100A6 using anti-S100A6 antibody.S100A6 was detected in paraffin-embedded section of Mouse Testis tissues.

IHC analysis of S100A6 using anti-S100A6 antibody.S100A6 was detected in paraffin-embedded section of Rat Lung tissues.