SIAH Interacting Protein/CACYBP Antibody

• Catalog Number: orb371640
• Category: Antibodies
• Description: SIAH Interacting Protein/CACYBP Antibody
• Species/Host: Rabbit
• Clonality: Polyclonal
• Tested applications: FC, ICC, IF, IHC, WB
• Predicted Reactivity: Bovine, Monkey
• Reactivity: Human, Mouse, Rat
• Isotype: Rabbit IgG
• Immunogen: A synthetic peptide corresponding to a sequence at the N-terminus of human CACYBP (7-47aa QKDLEEVKVLLEKATRKRVRDALTAEKSKIETEIKNKMQQK), different from the related mouse sequence by five amino acids, and from the related rat sequence by six amino acids.
• Concentration: Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.
• Dilution range: Western blot, 0.1-0.5μg/ml, Human, Mouse, Rat Immunohistochemistry (Paraffin-embedded Section), 0.5-1μg/ml, Human, Mouse, Rat, By Heat Immunocytochemistry/Immunofluorescence,2μg/ml, Human Flow Cytometry, 1-3μg/1x106 cells, Human
• Form/Appearance: Lyophilized
• Conjugation: Unconjugated
• MW: 26210 MW
• UniProt ID: Q9HB71
• Storage: Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.
• Alternative names: Calcyclin-binding protein;CacyBP;hCacyBP;S100A6-bi
• Note: For research use only
• Application notes: Tested Species: In-house tested species with positive results. By Heat: Boiling the paraffin sections in 10mM citrate buffer, pH6.0, for 20mins is required for the staining of formalin/paraffin sections. Other applications have not been tested. Optimal dilutions should be determined by end users. . Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
• Expiration Date: 12 months from date of receipt.

ICC analysis of CACYBP using anti-CACYBP antibody.CACYBP was detected in immunocytochemical section of MCF-7 cell.Biotinylated goat anti-rabbit IgG was used as secondary antibody.

WB analysis of CACYBP using anti-CACYBP antibody.Lane 1:human MCF-7 cell; 2:human RT4 cell; 3:human SW620 cell; 4:human U251 cell; 5:rat liver tissue; 6:rat brain tissue; 7:mouse liver tissue; 8:mouse brain tissue.

IHC analysis of CACYBP using anti-CACYBP antibody. CACYBP was detected in a paraffin-embedded section of human intestinal cancer tissue.

IHC analysis of CACYBP using anti-CACYBP antibody. CACYBP was detected in a paraffin-embedded section of mouse testis tissue.

IHC analysis of CACYBP using anti-CACYBP antibody. CACYBP was detected in a paraffin-embedded section of rat testis tissue.