RPSA Antibody / 40S Ribosomal protein SA

• Catalog Number: orb2642147
• Category: Antibodies
• Description: Required for the assembly and/or stability of the 40S ribosomal subunit. Required for the processing of the 20S rRNA-precursor to mature 18S rRNA in a late step of the maturation of 40S ribosomal subunits. Also functions as a cell surface receptor for laminin. Plays a role in cell adhesion to the basement membrane and in the consequent activation of signaling transduction pathways. May play a role in cell fate determination and tissue morphogenesis. Acts as a PPP1R16B-dependent substrate of PPP1CA. [UniProt]
• Species/Host: Mouse
• Clonality: Monoclonal
• Clone Number: LMNR-1
• Tested applications: FACS, IF, IHC-P, WB
• Reactivity: Human
• Isotype: Mouse IgG
• Immunogen: Recombinant full length protein was used as the immunogen for the RPSA antibody.
• Antibody Type: Primary Antibody
• Dilution range: Immunofluorescence: 1-2ug/ml,Flow cytometry: 1-2ug/million cells,Western blot: 1-2ug/ml,Immunohistochemistry (FFPE): 1-2ug/ml for 30 min at RT,Prediluted IHC only format: incubate for 30 min at RT (1)
• Purity: Protein G affinity chromatography
• Conjugation: Unconjugated
• Formula: 0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced) and 0.05% sodium azide
• Hazard Information: This RPSA antibody is available for research use only.
• UniProt ID: P08865
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Buffer/Preservatives: 0.2 mg/ml in 1X PBS with 0.1 mg/ml rAlbumin (US sourced) and 0.05% sodium azide
• Note: For research use only
• Application notes: The stated application concentrations are suggested starting points. Titration of the RPSA antibody may be required due to differences in protocols and secondary/substrate sensitivity.1. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.
• Expiration Date: 12 months from date of receipt.

Western blot testing of human HeLa cell lysate with RPSA antibody (clone LMNR-1). Routinely observed molecular weight: 37-40 kDa and 67 kDa.

IHC testing of FFPE human tonsil stained with RPSA antibody (clone LMNR-1). Required HIER: boiling tissue sections in pH9 10 mM Tris with 1 mM EDTA for 10-20 min followed by cooling at RT for 20 min.

IHC testing of FFPE human cervical carcinoma stained with RPSA antibody (clone LMNR-1). Required HIER: boiling tissue sections in pH9 10 mM Tris with 1 mM EDTA for 10-20 min followed by cooling at RT for 20 min.

Immunofluorescent staining of PFA-fixed human HeLa cells with RPSA antibody (green, clone LMNR-1) and Reddot nuclear stain (red).

FACS staining of PFA-fixed Raji cells with RPSA antibody (clone LMNR-1); Red = isotype control, Blue = RPSA antibody.