RPA32 Antibody

• Catalog Number: orb1524018
• Category: Antibodies
• Description: RPA32 Antibody
• Species/Host: Goat
• Clonality: Polyclonal
• Tested applications: IHC, WB
• Reactivity: Human
• Immunogen: Between 220 and 270
• Concentration: 1000 µg/ml
• Dilution range: WB - 1:500 - 1:2,500; IP - Not recommended; IHC - 1:500 - 1:2,000. Epitope retrieval with citrate buffer pH 6.0 or Tris-EDTA pH 9.0 is recommended for FFPE tissue sections.
• Form/Appearance: Whole IgG
• Conjugation: Unconjugated
• Target: RPA32
• UniProt ID: P15927
• NCBI: NP_002937.1
• Storage: 2 - 8°C
• Buffer/Preservatives: Tris-citrate/phosphate buffer, pH 7 to 8 containing 0.09% Sodium Azide
• Alternative names: replication factor A protein 2; RPA32; RP-A p34; R
• Note: For research use only
• Application notes: Format: Whole IgG
• Expiration Date: 12 months from date of receipt.

Detection of human RPA32 by western blot. Samples: Whole cell lysate from HEK293T (15 and 50 µg) , HeLa (50µg) , and Jurkat (50µg) cells. Antibodies: Affinity purified goat anti-RPA32 antibody orb1524018 used for WB at 1 µg/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.

Detection of human RPA32 by immunohistochemistry.Sample: FFPE section of human stomach carcinoma. Antibody: Affinity purified goat anti-RPA32 (Cat. No. orb1524018) used at a dilution of 1: 1, 000 (1µg/ml). Detection: DAB