RPA32 Antibody

• Catalog Number: orb1530481
• Category: Antibodies
• Description: RPA32 Antibody
• Species/Host: Rabbit
• Clonality: Polyclonal
• Tested applications: FC, IHC, IP, WB
• Reactivity: Human, Mouse
• Immunogen: between 225 and C-term
• Concentration: 1000 µg/ml
• Dilution range: WB - 1:2,000 - 1:10,000; IP - 2 - 10 µg/mg lysate; IHC - 1:2,000 to 1:10,000. Epitope retrieval with Tris-EDTA pH9.0 is recommended for FFPE tissue sections.
• Form/Appearance: Whole IgG
• Conjugation: Unconjugated
• Target: RPA32
• UniProt ID: P15927
• NCBI: NP_002937.1
• Storage: 2 - 8°C
• Buffer/Preservatives: Tris-citrate/phosphate buffer, pH 7 to 8 containing 0.09% Sodium Azide
• Alternative names: replication factor A protein 2; RPA32; RP-A p34; R
• Note: For research use only
• Application notes: Format: Whole IgG
• Expiration Date: 12 months from date of receipt.

Detection of human RPA32 by western blot.Samples: Whole cell lysate from HEK293T, HeLa, Jurkat, and U2OS cells. Antibody: Affinity purified rabbit anti-RPA32 antibody at 0.1 µg/ml.

Detection of mouse RPA32 by western blot.Samples: Whole cell lysate from CT26, CH27, TCMK-1, and BW5147.3 cells. Antibody: Affinity purified rabbit anti-RPA32 antibody at 0.04 µg/ml.

Detection of human RPA32 by immunohistochemistry. Sample: FFPE section of human breast carcinoma. Antibody: Affinity purified rabbit anti-RPA32 used at a dilution of 1:5, 000 (0.2 µg/ml).

Detection of mouse RPA32 by immunohistochemistry. Sample: FFPE section of mouse kidney. Antibody: Affinity purified rabbit anti-RPA32 used at a dilution of 1:5, 000 (0.2 µg/ml).

Detection of human RPA32 by western blot of immunoprecipitates.Samples: Whole cell lysate from HEK293T cells treated with 100 µM etoposide for 4 hours (+) or mock treated (-). Antibodies: Affinity purified rabbit anti-RPA32 antibody.