replication protein A2 Antibody

• Catalog Number: orb556832
• Category: Antibodies
• Description: Rabbit polyclonal antibody to RPA32
• Species/Host: Rabbit
• Clonality: Polyclonal
• Tested applications: ICC, IHC-P, WB
• Reactivity: Human, Mouse, Rat
• Isotype: IgG
• Immunogen: Recombinant protein encompassing a sequence within the center region of human RPA32. The exact sequence is proprietary.
• Concentration: 1 mg/ml
• Dilution range: WB: 1:500-1:3000 , IF: 1:100-1:1000 , IHC-P: 1:100-1:1000
• Form/Appearance: Liquid: 0.1M Tris, 0.1M Glycine, 10% Glycerol (pH7). 0.01% Thimerosal was added as a preservative.
• Purity: Purified by antigen-affinity chromatography.
• Conjugation: Unconjugated
• MW: 29
• Target: RPA32
• UniProt ID: P15927
• Storage: Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4°C. For long-term storage, aliquot and store at -20°lC or below. Avoid multiple freeze-thaw cycles.
• Buffer/Preservatives: 0.025% ProClin 300. 1XPBS pH 7, 20% Glycerol
• Alternative names: replication protein A2 , REPA2 , RP-A p32 , RP-A p
• Note: For research use only
• Application notes: Positive control: BCL-1 , PC-12 , Rat2
• Expiration Date: 12 months from date of receipt.

Immunofluorescence analysis of HeLa cells using RPA32 antibody

Immunohistochemical staining of A549 xenograft using RPA32 antibody

Western blot analysis of 293T(Lane 1), A431(Lane 2) using RPA32 antibody

Western blot analysis of 293T(Lane 1), A431(Lane 2) using RPA32 antibody

Western blot analysis of 293T(Lane 1), A431(Lane 2) using RPA32 antibody