ROBO1 Antibody

• Catalog Number: orb546314
• Category: Antibodies
• Description: ROBO1 Antibody
• Species/Host: Rabbit
• Clonality: Polyclonal
• Tested applications: ELISA, FC, ICC, IF, WB
• Reactivity: Human
• Isotype: Rabbit IgG
• Immunogen: E.coli-derived human ROBO1 recombinant protein (Position: F264-P897).
• Concentration: Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.
• Dilution range: Western blot, 0.1-0.5μg/ml Immunocytochemistry/Immunofluorescence, 2μg/ml Flow Cytometry, 1-3μg/1x106 cells Direct ELISA, 0.1-0.5μg/ml
• Form/Appearance: Lyophilized
• Conjugation: Unconjugated
• MW: 250 kDa
• UniProt ID: Q9Y6N7
• Storage: Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.
• Alternative names: Roundabout homolog 1; Deleted in U twenty twenty;
• Note: For research use only
• Application notes: Tested Species: In-house tested species with positive results. Other applications have not been tested. Optimal dilutions should be determined by end users. Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
• Expiration Date: 12 months from date of receipt.

Flow Cytometry analysis of PC-3 cells using anti-ROBO1 antibody (Blue line).Isotype control antibody (Green line) was rabbit IgG .Unlabelled sample (Red line) was also used as a control.

WB analysis of ROBO1 using anti-ROBO1 antibody.Lane 1:human U87 cell; 2:human HEK293 cell; 3:human HepG2 cell; 4:human A549 cell; 5:human PC-3 cell; 6:human HeLa cell.

IF analysis of ROBO1 using anti-ROBO1 antibody. ROBO1 was detected in immunocytochemical section of U20S cells.