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Product Overview
| Product Name | ROBO1 antibody |
|---|---|
| Catalog Number | orb19276 |
| Species/Host | Goat |
| Reactivity | Human |
| Predicted Reactivity | Bovine, Canine, Mouse, Rat |
| Conjugation | Unconjugated |
| Tested applications | ELISA, IHC-P, WB |
| Immunogen | Peptide with sequence C-GDVDLSNKINEMK, from the internal region of the protein sequence according to NP_002932.1. |
| Target | ROBO1 |
| Alternative Names | (click to expand) |
Product Properties
| Form/Appearance | Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin. |
|---|---|
| Storage | Aliquot and store at -20°C. Minimize freezing and thawing. |
| Note | For research use only. |
| Clonality | Polyclonal |
| Purity | Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide. |
| MW | 181 kDa |
| Uniprot ID | Q9Y6N7 (Human); O89026 |
| NCBI | NP_002932.1, NP_598334.1 |
| Entrez | 6091 |
Product Description
Goat polyclonal antibody to ROBO1
Application Notes
| Dilution Range | P-ELISA: 1:8000, WB: 1-3 μg/ml, IHC-P: 2 μg/ml |
|---|---|
| Application Notes | ELISA: Peptide ELISA: antibody detection limit dilution 1:8000. WB: Approx 250kDa band observed in Human Endothelial Cell line HUVEC lysates (calculated MW of 181kDa according to NP_002932.1). Some of the observed proteolytic breakdown products are also found in Human, Mouse and Rat Brain lysates. Recommended concentration: 1-3 μg/ml. |
Validation Images 
Western blot analysis of HUVEC lysate using ROBO1 antibody
Immunohistochemical staining of Human Liver using ROBO1 antibody
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