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Product Overview
Product Name ROBO1 antibody
Catalog Number orb19276
Species/HostGoat
ReactivityHuman
Predicted Reactivity Bovine, Canine, Mouse, Rat
ConjugationUnconjugated
Tested applicationsIHC-P, P-ELISA, WB
Immunogen Peptide with sequence C-GDVDLSNKINEMK, from the internal region of the protein sequence according to NP_002932.1.
Target ROBO1
Alternative Names
Product Properties
Form/Appearance Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Storage Aliquot and store at -20°C. Minimize freezing and thawing.
Note For research use only.
ClonalityPolyclonal
Purity Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
MW 181 kDa
Uniprot ID Q9Y6N7 (Human); O89026
NCBI NP_002932.1, NP_598334.1
Entrez 6091
Product Description

Goat polyclonal antibody to ROBO1

Application Notes
Dilution Range P-ELISA: 1:8000, WB: 1-3 μg/ml, IHC-P: 2 μg/ml
Application Notes ELISA: Peptide ELISA: antibody detection limit dilution 1:8000.

WB: Approx 250kDa band observed in Human Endothelial Cell line HUVEC lysates (calculated MW of 181kDa according to NP_002932.1). Some of the observed proteolytic breakdown products are also found in Human, Mouse and Rat Brain lysates. Recommended concentration: 1-3 μg/ml.
Validation Images
Western blot analysis of HUVEC lysate using ROBO1 antibody
Western blot analysis of HUVEC lysate using ROBO1 antibody
Immunohistochemical staining of Human Liver using ROBO1 antibody
Immunohistochemical staining of Human Liver using ROBO1 antibody
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