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Catalog Number | orb1265623 |
---|---|
Category | Antibodies |
Description | RNF31 Antibody |
Species/Host | Rabbit |
Clonality | Polyclonal |
Tested applications | WB |
Reactivity | Human |
Isotype | Rabbit Ig |
Immunogen | This RNF31 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 955-983 amino acids from the C-terminal region of human RNF31. |
Antibody Type | Primary Antibody |
Concentration | batch dependent |
Form/Appearance | Liquid |
Conjugation | Unconjugated |
MW | 120 kDa |
Target | RNF31 |
UniProt ID | Q96EP0 |
NCBI | Q96EP0 |
Storage | Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles. |
Buffer/Preservatives | Supplied in PBS with 0.09% (W/V) sodium azide. |
Alternative names | E3 ubiquitin-protein ligase RNF31, 632-, HOIL-1-in Read more... |
Note | For research use only |
Application notes | For WB starting dilution is: 1:2000 |
Expiration Date | 12 months from date of receipt. |
Western Blot at 1:2000 dilution Lane 1: 293T/17 whole cell lysate Lane 2: CCRF-CEM whole cell lysate Lane 3: Hela whole cell lysate Lane 4: Jurkat whole cell lysate Lane 5: MDA-MB-453 whole cell lysate Lane 6: MCF-7 whole cell lysate Lysates/proteins at 20 ug per lane.
Western Blot at 1:2000 dilution Lane 1: 293T/17 whole cell lysate Lane 2: CCRF-CEM whole cell lysate Lane 3: Hela whole cell lysate Lane 4: Jurkat whole cell lysate Lane 5: MDA-MB-453 whole cell lysate Lane 6: MCF-7 whole cell lysate Lysates/proteins at 20 ug per lane.
Western Blot at 1:2000 dilution Lane 1: 293T/17 whole cell lysate Lane 2: CEM whole cell lysate Lane 3: Hela whole cell lysate Lane 4: HepG2 whole cell lysate Lane 5: Jurkat whole cell lysate Lane 6: MCF-7 whole cell lysate Lysates/proteins at 20 ug per lane.
Western blot analysis in CEM cell line lysates (35 ug/lane).
ELISA, FC, ICC, IF, IHC, WB | |
Human, Mouse, Rat | |
Rabbit | |
Polyclonal | |
Unconjugated |
WB | |
Bovine, Canine, Equine, Guinea pig, Mouse, Rabbit, Rat | |
Human | |
Rabbit | |
Polyclonal | |
Unconjugated |
FC, ICC, IF, IHC-Fr, IHC-P | |
Canine, Equine, Porcine, Rabbit | |
Human, Mouse, Rat | |
Rabbit | |
Polyclonal | |
Unconjugated |
ELISA, WB | |
Canine, Human, Porcine | |
Goat | |
Polyclonal | |
Unconjugated |