RNF31 Rabbit Polyclonal Antibody

SKU: orb578765

Description

Rabbit polyclonal antibody to RNF31

Research Area

Epigenetics & Chromatin

Images & Validation

• Tested Applications: WB
• Reactivity: Human
• Predicted Reactivity: Bovine, Canine, Equine, Guinea pig, Mouse, Rabbit, Rat

Key Properties

• Host: Rabbit
• Clonality: Polyclonal
• Immunogen: The immunogen is a synthetic peptide directed towards the middle region of human RNF31
• Target: RNF31
• Protein Sequence: Synthetic peptide located within the following region: SLINAHSLDPATLYEVEELETATERYLHVRPQPLAGEDPPAYQARLLQKL
• Molecular Weight: 120kDa
• Purification: Affinity Purified
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Buffer/Preservatives: Liquid. Purified antibody supplied in 1x PBS buffer with 0.09% (w/v) sodium azide and 2% sucrose.
• Concentration: 0.5 mg/ml
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

HOIP, Paul, ZIBRA

Sample Tissue: Human MCF7, Antibody Dilution: 1.0 ug/ml.

Sample Type: Jurkat Whole Cell, Lane A: Primary Antibody, Lane B: Primary Antibody + Blocking Peptide, Primary Antibody Concentration: 1 ug/ml, Peptide Concentration: 5 ug/ml, Lysate Quantity: 25 ug/lane/Lane, Gel Concentration: 0.12.

Lanes: Lane 1: 50 ug hormoxia A549 lysate, Lane 2: 50 ug hypoxia A549 lysate, Lane 3: 50 ug hormoxia A549 lysate (+scrambled siRNA), Lane 4: 50 ug hypoxia A549 lysate (+scrambled siRNA), Lane 5: 50 ug hormoxia A549 lysate (RNF31 siRNA), Lane 6: 50 ug hypoxia A549 lysate (RNF31 siRNA), Primary Antibody Dilution: 1:800, Secondary Antibody: Goat anti rabbit HRP, Secondary Antibody Dilution: 1:10000, Gene Name: RNF31.

WB Suggested Anti-RNF31 Antibody Titration: 0.2-1 ug/ml, ELISA Titer: 1:312500, Positive Control: Jurkat cell lysate.

Quick Database Links

Gene Symbol

RNF31

UniProt

Q96EP0

RefSeq (Protein):NP_060469