RNF31 Rabbit Polyclonal Antibody

• Catalog Number: orb578765
• Category: Antibodies
• Description: Rabbit polyclonal antibody to RNF31
• Target: RNF31
• Clonality: Polyclonal
• Species/Host: Rabbit
• Conjugation: Unconjugated
• Reactivity: Human
• Predicted Reactivity: Bovine, Canine, Equine, Guinea pig, Mouse, Rabbit, Rat
• Form/Appearance: Liquid. Purified antibody supplied in 1x PBS buffer with 0.09% (w/v) sodium azide and 2% sucrose.
• Concentration: 0.5 mg/ml
• Buffer/Preservatives: Liquid. Purified antibody supplied in 1x PBS buffer with 0.09% (w/v) sodium azide and 2% sucrose.
• Purification: Affinity Purified
• Immunogen: The immunogen is a synthetic peptide directed towards the middle region of human RNF31
• Protein Sequence: Synthetic peptide located within the following region: SLINAHSLDPATLYEVEELETATERYLHVRPQPLAGEDPPAYQARLLQKL
• UniProt ID: Q96EP0
• MW: 120kDa
• Tested applications: WB
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Alternative names: HOIP, Paul, ZIBRA
• Research Area: Epigenetics & Chromatin
• Note: For research use only
• NCBI: NP_060469

Sample Tissue: Human MCF7, Antibody Dilution: 1.0 ug/ml.

Sample Type: Jurkat Whole Cell, Lane A: Primary Antibody, Lane B: Primary Antibody + Blocking Peptide, Primary Antibody Concentration: 1 ug/ml, Peptide Concentration: 5 ug/ml, Lysate Quantity: 25 ug/lane/Lane, Gel Concentration: 0.12.

Lanes: Lane 1: 50 ug hormoxia A549 lysate, Lane 2: 50 ug hypoxia A549 lysate, Lane 3: 50 ug hormoxia A549 lysate (+scrambled siRNA), Lane 4: 50 ug hypoxia A549 lysate (+scrambled siRNA), Lane 5: 50 ug hormoxia A549 lysate (RNF31 siRNA), Lane 6: 50 ug hypoxia A549 lysate (RNF31 siRNA), Primary Antibody Dilution: 1:800, Secondary Antibody: Goat anti rabbit HRP, Secondary Antibody Dilution: 1:10000, Gene Name: RNF31.

WB Suggested Anti-RNF31 Antibody Titration: 0.2-1 ug/ml, ELISA Titer: 1:312500, Positive Control: Jurkat cell lysate.