RNF3 antibody

• Catalog Number: orb18429
• Category: Antibodies
• Description: Goat polyclonal to RNF3
• Species/Host: Goat
• Clonality: Polyclonal
• Tested applications: ELISA, WB
• Reactivity: Bovine, Canine, Human, Mouse
• Dilution range: ELISA: 1:2000, WB: 0.5-2 μg/ml
• Conjugation: Unconjugated
• MW: 28.1
• Target: RNF3 (247 aa isoform)
• Entrez: 10336
• Protein Sequence: PLLLHYRPKMDLL
• Storage: Aliquot and store at -20°C. Minimize freezing and thawing.
• Buffer/Preservatives: Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.
• Alternative names: anti RNF3 antibody, anti DONG1 antibody, anti RNF3
• Note: For research use only
• Application notes: This antibody is expected to recognise the 247 aa isoform only (NP_006306), not the 132 aa isoform (NP_694982).ELISA: Peptide ELISA: antibody detection limit dilution 1:2000.WB: Approx 35kDa band seen in Human Heart lysate [Predicted MW of approx. 27kDa according to NP_006306]. Recommended for use at 0.5-2 μg/ml.
• Expiration Date: 12 months from date of receipt.

orb18429 staining (1ug/ml) of Human Heart lysate (RIPA buffer, 30ug total protein per lane). Primary incubated for 1 hour. Detected by western blot using chemiluminescence.