BRD2 Antibody

• Catalog Number: orb1526368
• Category: Antibodies
• Description: BRD2 Antibody
• Species/Host: Rabbit
• Clonality: Polyclonal
• Tested applications: IHC, IP, WB
• Reactivity: Human, Mouse
• Immunogen: between 675 and 725
• Concentration: 200 µg/ml
• Dilution range: WB - 1:2,000 - 1:10,000; IP - 2 - 10 µg/mg lysate; IHC - 1:100 to 1:500. Epitope retrieval with citrate buffer pH6.0 is recommended for FFPE tissue sections.
• Form/Appearance: Whole IgG
• Conjugation: Unconjugated
• Target: BRD2
• UniProt ID: P25440
• NCBI: NP_005095.1
• Storage: 2 - 8°C
• Buffer/Preservatives: Tris-buffered Saline containing 0.1% rAlbumin and 0.09% Sodium Azide
• Alternative names: D6S113E; RING3; really interesting new gene 3 prot
• Note: For research use only
• Application notes: Format: Whole IgG
• Expiration Date: 12 months from date of receipt.

Detection of mouse BRD2 by western blot. Samples: Whole cell lysate (50 µg) from RenCa and C2C12 cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-BRD2 antibody (orb1526368) used for WB at 0.04 µg/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.

Detection of human BRD2 by western blot of immunoprecipitates. Samples: Whole cell lysate (1.0 mg per IP reaction; 20% of IP loaded) from HEK293T cells prepared using NETN lysis buffer. Antibodies: Affinity purified rabbit anti-BRD2 antibody (orb1526368) used for IP at 6 µg per reaction. BRD2 was also immunoprecipitated by a previous lot of this antibody (orb1526368) and a second antibody against a different epitope of BRD2.

Detection of human BRD2 by immunohistochemistry.Sample: FFPE section of human skin carcinoma. Antibody: Affinity purified rabbit anti-BRD2 (orb1526368) used at a dilution of 1: 200 (1µg/ml). Detection: DAB

Detection of human BRD2 by western blot. Samples: Whole cell lysate (10 µg) from HeLa, MCF-7, Jurkat, Hep-G2, and HEK293T cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-BRD2 antibody (orb1526368) used for WB at 0.04 µg/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.