BRD2 Antibody

• Catalog Number: orb1526369
• Category: Antibodies
• Description: BRD2 Antibody
• Species/Host: Rabbit
• Clonality: Polyclonal
• Tested applications: ICC, IP, WB
• Reactivity: Human
• Immunogen: between 1 and 50
• Concentration: 1000 µg/ml
• Dilution range: WB - 1:2,000 - 1:10,000; IP - 2 - 10 µg/mg lysate; ICC-IF - 1:50 - 1:2,000. Formaldehyde fixation is recommended. Permeabilization with Triton-X 100 is recommended for formaldehyde-fixed cells.
• Form/Appearance: Whole IgG
• Conjugation: Unconjugated
• Target: BRD2
• UniProt ID: P25440
• NCBI: NP_005095.1
• Storage: 2 - 8°C
• Buffer/Preservatives: Tris-citrate/phosphate buffer, pH 7 to 8 containing 0.09% Sodium Azide
• Alternative names: D6S113E; RING3; really interesting new gene 3 prot
• Note: For research use only
• Application notes: Format: Whole IgG
• Expiration Date: 12 months from date of receipt.

Detection of human BRD2 by western blot. Samples: Whole cell lysate (50 µg) from HeLa, HEK293T, and Jurkat cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-BRD2 antibody orb1526369 used for WB at 0.1 µg/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.

Detection of human BRD2 by western blot of immunoprecipitates. Samples: Whole cell lysate (1.0 mg per IP reaction; 20% of IP loaded) from HEK293T cells prepared using NETN lysis buffer. Antibodies: Affinity purified rabbit anti-BRD2 antibody orb1526369 used for IP at 6 µg per reaction.

Detection of human BRD2 by western blot of immunoprecipitates. Samples: Whole cell lysate (1.0 mg per IP reaction; 20% of IP loaded) from HEK293T cells prepared using NETN lysis buffer. Antibodies: Affinity purified rabbit anti-BRD2 antibody orb1526369 used for IP at 6 µg per reaction.

Detection of human BRD2 by immunocytochemistry.Sample: Formaldehyde-fixed asynchronous HeLa cells. Antibody: Affinity purified rabbit anti-BRD2 (Cat. No.orb1526369) used at a dilution of 1: 100 (2µg/ml). Detection: Red-fluorescent goat anti-rabbit IgG cross-adsorbed Antibody DyLight 594 used at a dilution of 1: 100.