RETNLB antibody

• Catalog Number: orb19920
• Category: Antibodies
• Description: Goat polyclonal antibody to RETNLB
• Species/Host: Goat
• Clonality: Polyclonal
• Tested applications: ELISA, IHC
• Reactivity: Human
• Dilution range: ELISA: 1:128000, WB: 0.1 μg/ml, IHC-P: 2-4µg/ml
• Conjugation: Unconjugated
• MW: 11.7
• Target: RELMbeta
• Entrez: 84666
• Protein Sequence: DSVMDKKIKDVLNS
• Storage: Aliquot and store at -20°C. Minimize freezing and thawing.
• Buffer/Preservatives: Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.
• Alternative names: RETNLB , resistin like beta , FIZZ1, FIZZ2, HXCP2,
• Note: For research use only
• Application notes: ELISA: Peptide ELISA: antibody detection limit dilution 1:128000.IHC: In paraffin embedded Human Colon shows textured staning of cytoplasm in enterocytes. Recommended concentration, 2-4ug/ml.WB: Preliminary experiments gave an approx 35kDa band in Human Ileum and Duodenum lysates after 0.1 μg/ml antibody staining. Please note that currently we cannot find an explanation in the literature for the band we observe given the calculated size of 11.7kDa according to NP_115968.1. The 35kDa band was successfully blocked by incubation with the immunizing peptide. We would appreciate any feedback from people in the field - have any results been reported with other antibodies/lysates Have any further splice variants/modified forms been reported
• Expiration Date: 12 months from date of receipt.

orb19920 (2.5g/ml) staining of paraffin embedded Human Colon. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.