RETNLB Antibody

• Catalog Number: orb1272785
• Category: Antibodies
• Description: RETNLB Antibody
• Species/Host: Rabbit
• Clonality: Polyclonal
• Tested applications: ELISA, WB
• Reactivity: Human
• Immunogen: Produced from sera of rabbits pre-immunized with highly pure (>98%) recombinant mRELMb. Human RELMb specific antibody was purified by affinity chromatography employing immobilized RELMb matrix.
• Concentration: batch dependent
• Dilution range: ELISA:Indirect:To detect hRELMb by indirect ELISA (using 100 μL/well antibody solution) a concentration of 0.5 - 2.0 μg/mL of this antibody is required. This antigen affinity purified antibody, in conjunction with compatible secondary reagents, allows the detection of at least 0.2 - 0.4 ng/well of recombinant hRELMb. Sandwich:To detect hRELMb by sandwich ELISA (using 100 μL/well antibody solution) a concentration of 0.5 - 2.0 μg/mL of this antibody is required. Western Blot:To detect hRELMb by Western Blot analysis this antibody can be used at a concentration of 0.1 - 0.2 μg/mL. Used in conjunction with compatible secondary reagents the detection limit for recombinant hRELMb is 1.5 - 3.0 ng/lane, under either reducing or non-reducing conditions.
• Form/Appearance: Lyophilized
• Conjugation: Unconjugated
• Target: RETNLB
• UniProt ID: Q9BQ08
• NCBI: Q9BQ08
• Storage: RELMb antibody is stable for at least 2 years from date of receipt at -20°C. The reconstituted antibody is stable for at least two weeks at 2-8°C. Frozen aliquots are stable for at least 6 months when stored at -20°C. Avoid repeated freeze-thaw cycles.
• Alternative names: XCP2, FIZZ1, FIZZ2, HXCP2, RELMb, RELMbeta, RELM-b
• Note: For research use only
• Application notes: ELISA:Indirect:To detect hRELMb by indirect ELISA (using 100 μL/well antibody solution) a concentration of 0.5 - 2.0 μg/mL of this antibody is required. This antigen affinity purified antibody, in conjunction with compatible secondary reagents, allows the detection of at least 0.2 - 0.4 ng/well of recombinant hRELMb. Sandwich:To detect hRELMb by sandwich ELISA (using 100 μL/well antibody solution) a concentration of 0.5 - 2.0 μg/mL of this antibody is required. Western Blot:To detect hRELMb by Western Blot analysis this antibody can be used at a concentration of 0.1 - 0.2 μg/mL. Used in conjunction with compatible secondary reagents the detection limit for recombinant hRELMb is 1.5 - 3.0 ng/lane, under either reducing or non-reducing conditions.
• Expiration Date: 12 months from date of receipt.

To detect Human RELM-beta by sandwich ELISA (using 100 ul/well antibody solution) a concentration of 0.5 - 2.0 ug/ml of this antibody is required. This antigen affinity purified antibody, in conjunction with Biotinylated Anti-Human RELM-beta (orb1272786) as a detection antibody, allows the detection of at least 0.2 - 0.4 ng/well of recombinant Human RELM-beta.

To detect Human RELM-beta by Western Blot analysis this antibody can be used at a concentration of 0.1 - 0.2 ug/ml. When used in conjunction with compatible secondary reagents, the detection limit for recombinant Human RELM-beta is 1.5 - 3.0 ng/lane, under either reducing or non-reducing conditions.

This antibody stained formalin-fixed, paraffin-embedded sections of human colon/rectum adenocarcinoma. The recommended concentration is 1.0 ug/ml with an overnight incubation at 4 °C. An HRP-labeled polymer detection system was used with a DAB chromogen. Optimal results for these conditions were achieved without antigen retrieval. Optimal concentrations and conditions may vary.