Retnlb Antibody

• Catalog Number: orb1272789
• Category: Antibodies
• Description: Retnlb Antibody
• Species/Host: Rabbit
• Clonality: Polyclonal
• Tested applications: ELISA, WB
• Reactivity: Mouse
• Immunogen: Produced from sera of rabbits pre-immunized with highly pure (>98%) recombinant mRELMb. Murine RELMb specific antibody was purified by affinity chromatography employing immobilized RELMb matrix.
• Concentration: batch dependent
• Dilution range: ELISA:Indirect:To detect mRELMb by indirect ELISA (using 100 μL/well antibody solution) a concentration of 0.5 - 2.0 μg/mL of this antibody is required. This antigen affinity purified antibody, in conjunction with compatible secondary reagents, allows the detection of at least 0.2 - 0.4 ng/well of recombinant mRELMb. Sandwich:To detect mRELMb by sandwich ELISA (using 100 μL/well antibody solution) a concentration of 0.5 - 2.0 μg/mL of this antibody is required.Western Blot:To detect mRELMb by Western Blot analysis this antibody can be used at a concentration of 0.1 - 0.2 μg/mL. Used in conjunction with compatible secondary reagents the detection limit for recombinant mRELMb is 1.5 - 3.0 ng/lane, under either reducing or non-reducing conditions.
• Form/Appearance: Lyophilized
• Conjugation: Unconjugated
• Target: Retnlb
• UniProt ID: Q99P86
• NCBI: Q99P86
• Storage: RELMb antibody is stable for at least 2 years from date of receipt at -20°C. The reconstituted antibody is stable for at least two weeks at 2-8°C. Frozen aliquots are stable for at least 6 months when stored at -20°C. Avoid repeated freeze-thaw cycles.
• Alternative names: Xcp3, Fizz2, Relmb, RELMbeta, 9030012B21Rik, Resis
• Note: For research use only
• Application notes: ELISA:Indirect:To detect mRELMb by indirect ELISA (using 100 μL/well antibody solution) a concentration of 0.5 - 2.0 μg/mL of this antibody is required. This antigen affinity purified antibody, in conjunction with compatible secondary reagents, allows the detection of at least 0.2 - 0.4 ng/well of recombinant mRELMb. Sandwich:To detect mRELMb by sandwich ELISA (using 100 μL/well antibody solution) a concentration of 0.5 - 2.0 μg/mL of this antibody is required.Western Blot:To detect mRELMb by Western Blot analysis this antibody can be used at a concentration of 0.1 - 0.2 μg/mL. Used in conjunction with compatible secondary reagents the detection limit for recombinant mRELMb is 1.5 - 3.0 ng/lane, under either reducing or non-reducing conditions.
• Expiration Date: 12 months from date of receipt.

To detect Murine RELM-beta by sandwich ELISA (using 100 ul/well antibody solution) a concentration of 0.5 - 2.0 ug/ml of this antibody is required. This antigen affinity purified antibody, in conjunction with Biotinylated Anti-Murine RELM-beta (orb1272793) as a detection antibody, allows the detection of at least 0.2 - 0.4 ng/well of recombinant Murine RELM-beta.

To detect Murine RELM-beta by Western Blot analysis this antibody can be used at a concentration of 0.1 - 0.2 ug/ml. when used in conjunction with compatible secondary reagents, the detection limit for recombinant Murine RELM-beta is 1.5 - 3.0 ng/lane, under either reducing or non-reducing conditions.

To detect Murine RELM-beta by Western Blot analysis this antibody can be used at a concentration of 0.1 - 0.2 ug/ml. when used in conjunction with compatible secondary reagents, the detection limit for recombinant Murine RELM-beta is 1.5 - 3.0 ng/lane, under either reducing or non-reducing conditions.

This antibody stained 4% PFA, paraffin-embedded sections of murine cecum tissue (with positive goblet cells of a Trichuris muris infected mouse). The primary antibody was incubated at a concentration of 0.33 ng/mL overnight at 4 °C and the secondary antibody was a cyanine-2 conjugated Donkey anti-Rabbit (Jackson ImmunoResearch). Heat induced antigen retrieval with a 100mM Citric Acid was used. Optimal concentrations and conditions may vary.