Resistin Antibody / RETN

• Catalog Number: orb2637225
• Category: Antibodies
• Description: The cysteine-rich, adipose tissue-specific, secretory factor resistin (resistance to Insulin) also known as ADSF, is a secreted hormone that potentially links obesity to diabetes. Resistin is rich in serine and cysteine residues and contains a unique cysteine repeat motif. Resistin and the resistin-like molecules share the characteristic cysteine composition and other signature features. Resistin-like expression is highest in proliferative epithelial cells and is markedly increased in tumors, suggesting a role in intestinal proliferation.
• Species/Host: Mouse
• Clonality: Monoclonal
• Clone Number: RETN/4326
• Tested applications: IHC-P, WB
• Reactivity: Human
• Isotype: Mouse IgG2b, kappa
• Immunogen: A portion of amino acids 19-108 was used as the immunogen for the RETN antibody.
• Antibody Type: Primary Antibody
• Dilution range: Western blot: 2-4 ug/ml,Immunohistochemistry (FFPE): 1-2ug/ml
• Purity: Protein A/G affinity
• Conjugation: Unconjugated
• Formula: 0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced), 0.05% sodium azide
• Hazard Information: This RETN antibody is available for research use only.
• UniProt ID: Q9HD89
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Buffer/Preservatives: 0.2 mg/ml in 1X PBS with 0.1 mg/ml rAlbumin (US sourced), 0.05% sodium azide
• Note: For research use only
• Application notes: Optimal dilution of the RETN antibody should be determined by the researcher.
• Expiration Date: 12 months from date of receipt.

IHC staining of FFPE human spleen tissue with RETN antibody (clone RETN/4326). HIER: boil tissue sections in pH9 10mM Tris with 1mM EDTA for 20 min and allow to cool before testing.

IHC staining of FFPE human spleen tissue with RETN antibody (clone RETN/4326). HIER: boil tissue sections in pH9 10mM Tris with 1mM EDTA for 20 min and allow to cool before testing.

Western blot testing of human heart tissue lysate using RETN antibody (clone RETN/4326). Predicted molecular weight ~11 kDa.

Analysis of HuProt (TM) microarray containing more than 19000 full-length human proteins using RETN antibody (clone RETN/4326). These results demonstrate the foremost specificity of the RETN/4326 mAb. Z- and S- score: The Z-score represents the strength of a signal that an antibody (in combination with a fluorescently-tagged anti-IgG secondary Ab) produces when binding to a particular protein on the HuProt (TM) array. Z-scores are described in units of standard deviations (SD's) above the mean value of all signals generated on that array. If the targets on the HuProt (TM) are arranged in descending order of the Z-score, the S-score is the difference (also in units of SD's) between the Z-scores. The S-score therefore represents the relative target specificity of an Ab to its intended target.