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Catalog Number | orb2635798 |
---|---|
Category | Antibodies |
Description | The cysteine-rich, adipose tissue-specific, secretory factor resistin (resistance to Insulin) also known as ADSF, is a secreted hormone that potentially links obesity to diabetes. Resistin is rich in serine and cysteine residues and contains a unique cysteine repeat motif. Resistin and the resistin-like molecules share the characteristic cysteine composition and other signature features. Resistin-like alpha is a secreted protein that has restricted tissue distribution and is most highly expressed in adipose tissue. Another family member, resistin-like beta, is a secreted protein expressed only in the gastrointestinal tract, particularly in the colon, in both mouse and human. Resistin-like beta expression is highest in proliferative epithelial cells and is markedly increased in tumors, suggesting a role in intestinal proliferation. |
Species/Host | Mouse |
Clonality | Monoclonal |
Clone Number | RETN/4325 |
Tested applications | IHC-P, WB |
Reactivity | Human |
Isotype | Mouse IgG2, lambda |
Immunogen | A recombinant fragment of human RETN protein (within amino acids 19-108) was used as the immunogen for the Resistin antibody. |
Antibody Type | Primary Antibody |
Dilution range | Immunohistochemistry (FFPE): 1-2ug/ml,Western blot: 2-4ug/ml |
Conjugation | Unconjugated |
Formula | 1 mg/ml in 1X PBS; BSA free, sodium azide free |
Hazard Information | This Resistin antibody is available for research use only. |
UniProt ID | Q9HD89 |
Storage | Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles. |
Note | For research use only |
Application notes | Optimal dilution of the Resistin antibody should be determined by the researcher. |
Expiration Date | 12 months from date of receipt. |
IHC staining of FFPE human spleen tissue with Resistin antibody (clone RETN/4325). Inset: PBS used in place of primary Ab (secondary Ab negative control). HIER: boil tissue sections in pH9 10 mM Tris with 1 mM EDTA for 20 min and allow to cool before testing.
Western blot analysis of human heart tissue lysate using Resistin antibody (clone RETN/4325). Predicted molecular weight ~11 kDa.
SDS-PAGE analysis of purified, BSA-free Resistin antibody (clone RETN/4325) as confirmation of integrity and purity.