Resistin Antibody / RETN

• Catalog Number: orb2635799
• Category: Antibodies
• Description: The cysteine-rich, adipose tissue-specific, secretory factor resistin (resistance to Insulin) also known as ADSF, is a secreted hormone that potentially links obesity to diabetes. Resistin is rich in serine and cysteine residues and contains a unique cysteine repeat motif. Resistin and the resistin-like molecules share the characteristic cysteine composition and other signature features. Resistin-like alpha is a secreted protein that has restricted tissue distribution and is most highly expressed in adipose tissue. Another family member, resistin-like beta, is a secreted protein expressed only in the gastrointestinal tract, particularly in the colon, in both mouse and human. Resistin-like beta expression is highest in proliferative epithelial cells and is markedly increased in tumors, suggesting a role in intestinal proliferation.
• Species/Host: Mouse
• Clonality: Monoclonal
• Clone Number: RETN/4325
• Tested applications: IHC-P, WB
• Reactivity: Human
• Isotype: Mouse IgG2, lambda
• Immunogen: A recombinant fragment of human RETN protein (within amino acids 19-108) was used as the immunogen for the Resistin antibody.
• Antibody Type: Primary Antibody
• Dilution range: Immunohistochemistry (FFPE): 1-2ug/ml,Western blot: 2-4ug/ml
• Conjugation: Unconjugated
• Formula: 0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced), 0.05% sodium azide
• Hazard Information: This Resistin antibody is available for research use only.
• UniProt ID: Q9HD89
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Note: For research use only
• Application notes: Optimal dilution of the Resistin antibody should be determined by the researcher.
• Expiration Date: 12 months from date of receipt.

IHC staining of FFPE human spleen tissue with Resistin antibody (clone RETN/4325). Inset: PBS used in place of primary Ab (secondary Ab negative control). HIER: boil tissue sections in pH9 10 mM Tris with 1 mM EDTA for 20 min and allow to cool before testing.

Western blot analysis of human heart tissue lysate using Resistin antibody (clone RETN/4325). Predicted molecular weight ~11 kDa.

SDS-PAGE analysis of purified, BSA-free Resistin antibody (clone RETN/4325) as confirmation of integrity and purity.