RBX1 Antibody

• Catalog Number: orb1265034
• Category: Antibodies
• Description: RBX1 Antibody
• Species/Host: Rabbit
• Clonality: Polyclonal
• Tested applications: IF, IHC-P, WB
• Predicted Reactivity: C. elegans, Mouse
• Reactivity: Human
• Isotype: Rabbit Ig
• Immunogen: This RBX1 antibody is generated from a rabbit immunized with a KLH conjugated synthetic peptide between 74-108 amino acids from the C-terminal region of human RBX1.
• Concentration: batch dependent
• Dilution range: For WB starting dilution is: 1:1000For IHC-P starting dilution is: 1:25For IF starting dilution is: 1:25
• Form/Appearance: Liquid
• Conjugation: Unconjugated
• MW: 12 kDa
• Target: RBX1
• UniProt ID: P62877
• NCBI: P62877
• Storage: Store at 4°C for three months and -20°C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
• Buffer/Preservatives: Supplied in PBS with 0.09% (W/V) sodium azide.
• Alternative names: E3 ubiquitin-protein ligase RBX1, 632-, Protein ZY
• Note: For research use only
• Application notes: For WB starting dilution is: 1:1000For IHC-P starting dilution is: 1:25For IF starting dilution is: 1:25
• Expiration Date: 12 months from date of receipt.

Western Blot at 1:1000 dilution Lane 1: HepG2 whole cell lysate Lane 2: 293 whole cell lysate Lysates/proteins at 20 ug per lane.

Immunohistochemical analysis of paraffin-embedded M. testis section using RBX1 Antibody. Antibody was diluted at 1:100 dilution. A peroxidase-conjugated goat anti-rabbit IgG at 1:400 dilution was used as the secondary antibody, followed by DAB staining.

Fluorescent image of HeLa cells stained with RBX1 Antibody. Antibody was diluted at 1:25 dilution. An Alexa Fluor 488-conjugated goat anti-rabbit lgG at 1:400 dilution was used as the secondary antibody (green). Cytoplasmic actin was counterstained with Alexa Fluor 555 conjugated with Phalloidin (red).

Immunohistochemical analysis of paraffin-embedded H. colon section using RBX1 Antibody. Antibody was diluted at 1:100 dilution. A peroxidase-conjugated goat anti-rabbit IgG at 1:400 dilution was used as the secondary antibody, followed by DAB staining.

Western blot analysis of lysates from Hela, MCF-7 cell line (from left to right), using RBX1 Antibody at 1:1000 at each lane.